AKTIVITAS ANTIBAKTERI ISOLAT AKTINOMISETES DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli

Abstract

The use of antibiotics for infections treatment in the long term may cause bacteria become resistant. Exploration of microorganisms capable to produce antimicrobial compounds have widely been reported. One of which is actinomycetes, a gram-positive bacteria that produce various secondary metabolites. The purpose of this study was to test and select the ability of 20 isolates of actinomycetes (14 isolates from the rhizosphere of the Siak-siak plant (Dianella ensifolia) and six isolates from mangrove soil in Kuala Enok, Tanah Merah district, Indragiri Hilir) using plug agar diffusion method against E.coli under Mueller Hinton Agar (MHA) medium for 24 hours. Two isolates (RK4 and RW5) formed inhibition zones against E.coli with diameters of 8 mm and 10.5 mm, respectively. Based on the results obtained, the two isolates were classified as bacteriostatic. In addition, inhibition capability of RK4 and RW5 isolates were respectively classified into weak and moderate inhibitory criteria.