ISOLASI METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK n-HEKSANA DARI DAUN BAKAU Rhizopora mucronata (Rhizophoraceae)

Abstract

Rhizophora mucronata is type of mangrove that has diverse secondary metabolites, such us alkaloids, flavonoids, tannins and phenol compounds. The purpose of this study was to isolate secondary metabolites and to determine toxicity test by BSLT method. Leave extraction was carried out using ethanol and then fractionated with n-hexane. The fractionation produced 40 g dark green. Crude n-hexane extract was separated using liquid vacuum chromatography and 6 fractions produced. White crystals were isolated from second fraction of n-hexane with melting poin of 236-238 oC. The crystals were characterized using UV and FT-IR spectroscopy. UV spectrum of RmHF2 indicated absorption at λmax 292 nm. The FT-IR absorption spectrum showed absorption peaks at 1031 (C-O),1376 (C-H) methyl, 1463 (C-H) methylen,1605 (C=C), 1697 (C=O) dan 2942 cm-1 (CH aliphatic). The result of toxicity test by BSLT (Brine Shrimps Lethality Test) method on that crude extract was non-toxic with LC50 value of 841395 ppm.