Browsing by Author "Sitompul, Lorena Omega"

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    ANALISIS AKTIVITAS ENZIM AMILASE PRODUKSI JAMUR TERMOFILIK Aspergillus sp. LBKURCC304 DENGAN SUMBER KARBON BERBEDA
    (perpustakaan UR, 2021-08) Sitompul, Lorena Omega; Saryono, Saryono; Devi, Silvera
    Thermophilic amylase is great demand in industrial processes and biotechnology. The production of amylase can be affected by carbon sources. In this study, the production of amylase was carried out by Aspergillus sp. LBKURCC304 with several natural carbon sources (cassava, corn, taro, purple sweet potato, potato, breadfruit, canna, gembili, gadung, and sago). Production was carried out in submerged fermentation pH 7 at 50°C for 11 days with an agitation speed of 150 rpm. Determination of amylase activity was using the Nelson-Somogyi method, absorbance was measured using a UVVis spectrophotometer with a wavelength of 540 nm. The results showed that the highest activity was obtained in presence of sago carbohydrates (0.0391 ± 0.0017 U/mL) as a carbon source