Browsing by Author "Roza, Rodesia Mustika"
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Item AKTIVITAS ANTIBAKTERI AKTINOMISETES LAHAN GAMBUT RIMBO PANJANG KAMPAR RIAU TERHADAP Staphylococcus aureus(2016-05-19) Rosandi, Gita Putri; Roza, Rodesia Mustika; Martina, AtriaPotential actinomycetes in producing antibacterial compound has been widely studied. However, not so many information about antibacterial compound produced by local isolates actinomycetes from Riau despite its high diversity. The aim of this research is to determine potential of actinomycetes from Rimbo Panjang Peat, Kampar, Riau in producing antibacterial compounds against S. aureus. Agar disc method was used to test the antibacterial activity by measuring the inhibition zone. Furthermore, antibacterial compounds produced by isolated filtrate of actinomycetes, following five day fermentation, were tested using paper disc method. The result showed that 13 of 40 isolates which were tested using agar disc method had antibacterial activity against S. aureus with the highest inhibition zone was 18,45 mm (isolates KN 5.4). Meanwhile, only 6 of 13 isolates filtrate which were tested using paper disc method showed antibacterial activity with the highest inhibition zone of 16,16 mm (KN 5.4).Item AKTIVITAS ANTIBAKTERI EKSTRAK DAUN BENALU (Scurulla sp) YANG TUMBUH PADA BEBERAPA INANG TERHADAP PERTUMBUHAN Salmonella typhi(2013-03-04) Nasution, Pebriana; Roza, Rodesia Mustika; FitmawatiScurulla sp as arboreal parasitic plant that hold and absorb its nutrient from host. During nutrient absorption, all of metabolites (include secondary metabolites) which produce by the host will be also absorbed. For this reason Scurulla sp must have capability to develop strategies to adapt. Different host are estimated to produce different secondary metabolite. The aims of this study is to know the best solvent between water and methanol to reduce the antibacterial activity of leaf extracts Scurulla sp from different hosts (Coffea arabica, Theobroma cacao, Diospyros nigra) and to know the concentration exctract of Scurulla sp against S. typhi. The method used in this research is the experimental method in the laboratory. There are 5 concentration variations. This extraction performed used methanol and water, and were used series of dilution 10%, 25%, 50%, 75%, and 100%. The exctract than was tested by paper disc diffusion and agar wells method, then incubated for 24-72 hours at room temperature. Observations were made by measuring the area which of inhibition. The results showed the inhibition of the solvent methanol has the inhibition area is bigger than the solvent water at all concentrations. Extract of Scurulla sp from Coffea arabica host, Theobroma cacao host and Diospyros nigra host showed the highest in a 100% concentration. The lowest concentration of 10% is characterized by no area of the inhibition on all hosts with the methanol and water solventsItem AKTIVITAS ANTIBAKTERI EKSTRAK DAUN MAHANG (Macaranga triloba (Muell.) Arg.) TERHADAP Escherichia coli DAN Salmonella typhi(2013-03-04) Sari, Rika Purnama; Roza, Rodesia Mustika; FitmawatiMany kinds of plant species grow in Indonesia. One of them is Mahang (Macaranga triloba (Muell.) Arg.). Mahang (Macaranga triloba (Muell.) Arg.) leaf are often used as traditional medicine. The aims of this study were to find the activity of bacterias using 4 extraction methods and to find the most effective extraction method and concentration in inhibition the bacteria growth againts E. coli and S. typhi. The extraction which had been done by maceration method with methanol dissolver, boiling dry leaf, grinding leaf, boiling fresh leaf. The testing activity was done using paper disk and agar well by extract concentration 100%, 75%, 50%, 25% and 10%. The result of this research showed that of the most effective extraction method and concentration in producing inhibition zone were methanol in concentration of 100%, both E. coli and S. typhi, that is 15,43±2,19 mm and 20,67±1,14 mmItem AKTIVITAS ANTIBAKTERI ISOLAT AKTINOMISETES DALAM MENGHAMBAT PERTUMBUHAN Escherichia coli(Elfitra, 2022-04) Yahani, Imel; Roza, Rodesia MustikaThe use of antibiotics for infections treatment in the long term may cause bacteria become resistant. Exploration of microorganisms capable to produce antimicrobial compounds have widely been reported. One of which is actinomycetes, a gram-positive bacteria that produce various secondary metabolites. The purpose of this study was to test and select the ability of 20 isolates of actinomycetes (14 isolates from the rhizosphere of the Siak-siak plant (Dianella ensifolia) and six isolates from mangrove soil in Kuala Enok, Tanah Merah district, Indragiri Hilir) using plug agar diffusion method against E.coli under Mueller Hinton Agar (MHA) medium for 24 hours. Two isolates (RK4 and RW5) formed inhibition zones against E.coli with diameters of 8 mm and 10.5 mm, respectively. Based on the results obtained, the two isolates were classified as bacteriostatic. In addition, inhibition capability of RK4 and RW5 isolates were respectively classified into weak and moderate inhibitory criteria.Item AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER AKTINOMISETES YANG DIEKSTRAKSI MENGGUNAKAN ETIL ASETAT(2020-08) Melindah, Tamina; Roza, Rodesia MustikaInfection from pathogenic bacteria can cause various diseases in humans, such as diarrhea. The diarrheal disease can be caused by bacteria Escherichia coli, Salmonella typhimurium, and Shigella flexineri. Meanwhile, actinomycetes are reported to be one of the producers of antibiotics. The actinomycetes used were isolated from soil in the Universitas Riau Arboretum, namely isolates B1.06, B3.13, C2.18, A2.01, and C2.20. The purpose of this study was to study the antibacterial activity of secondary metabolites of Actinomycetes extracted using ethyl acetate against pathogenic bacteria that cause diarrheal diseases. The disk diffusion method was used for testing antibacterial activity. From the results, it was found that the actinomycetes isolate was able to overcome the pathogenic bacteria, with a diameter of inhibition zone formed was 8.2 mm against Salmonella typhimurium and 20.6 mm against Shigella flexineri. The diameter of the inhibition zone formed was compared with the tetracylcline and produced antibacterial activity criteria of actinomycetes isolates that were resistant, intermediate and sensitive.Item Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Alam Giam Siak Kecil-Bukit Batu Riau Terhadap Bakteri(wahyu sari yeni, 2017-09-13) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, AtriaPenelitian ini bertujuan untuk mendapatkan isolat aktinomisetes asal tanah gambut Cagar Alam Giam-Siak Kecil Bukit Batu Riau yang memiliki kemampuan daya hambat terhadap bakteri. Penelitian ini dilaksanakan di lapangan dan di laboratorium Mikrobiologi Jurusan FMIPA Universitas Riau mulai bulan Juli-Desember 2009. Lokasi pengambilan sampel terdiri atas empat lokasi yaitu : Lokasi 1 (Perkebunan Sawit dan Karet), Lokasi 2 (Hutan Tanaman Industri), Lokasi 3 (Hutan Paska Kebakaran) dan Lokasi 4 (Zona Inti). Penghitungan total mikroba dilakukan pada medium PCA (Plate Count Agar). Hasil penghitungan total mikroba diperoleh jumlah tertinggi pada lokasi 4 (zona inti) sebanyak 143 x 103 CFU /gram sampel tanah gambut dan terendah pada Lokasi 3 (Hutan Paska Kebakaran) sebanyak 39 x 103 CFU /gram sampel tanah gambut. Hasil isolasi aktinomisetes diperoleh total 22 isolat aktinomisetes, 2 isolat dari Lokasi 1 (Perkebunan Sawit dan Karet), 2 isolat dari Lokasi 2 (Hutan Tanaman Industri), 13 isolat dari Lokasi 3 (Hutan Paska Kebakaran) dan 5 isolat dari Lokasi 4 (Zona Inti). Warna koloni yang berhasil diisolasi dikelompokkan putih, krem, coklat, putih kecoklatan coklat kekuningan, dan kehijau-hijauan, permukaan bertepung, hampir keseluruhan berbentuk bulat, semua berkonsistensi lengket dan mengeluarkan bau serasah/ bau tanah. Isolasi dilakukan dengan menggunakan medium SCA (Starch Casein Agar). Pengujian kemampuan daya hambat terhadap bakteri uji (E.coli) diperoleh satu isolat (L3.9) memperlihatkan zona bening. Zona hambat yang terbentuk berdiameter 13,7 mm. Pengujian daya hambat terhadap bakteri dilakukan pada medium NA (Natrium Agar)Item Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Biosfir Giam Siak Kecil-Bukit Batu Riau Terhadap Bakteri Dan Jamur(wahyu sari yeni, 2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Fahrizawati, FahrizawatiCagar Biosfir Giam Siak Kecil-Bukit (Cagar Biosfir GSK-BB) merupakan hutan gambut dataran rendah. Tanah gambut ini merupakan habitat utama mikroba tersterial salah satunya aktinomisetes. Aktinomisetes memiliki kemampuan menghasilkan berbagai metabolit sekunder. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengeksplorasi aktinomisetes asal tanah gambut Cagar Biosfir GSK-BB dan melakukan uji daya hambat aktinomisetes terhadap bakteri dan jamur. Sebanyak 33 isolat aktinomisetes yang diisolasi dari sampel tanah gambut menggunakan metode pour plate dalam medium Strach Casein Agar, 2 isolat (GSK.5.7 dan GSK.5.10) mampu menghambat S. pyogenes dan 1 isolat (GSK.4.9) mampu menghambat E. Coli, 2 Isolat (GSK.4.3 dan GSK.4.5) mampu menghambat pertumbuhan F. oxysporum, dan 11 isolat (GSK.1.1, GSK.4.1, GSK.4.4, GSK.4.6, GSK.4.7, GSK.4.8, GSK.4.10, GSK.4.12, GSK.5.5, GSK.5.6, dan GSK.5.9) mampu menghambat pertumbuhan Ganoderma sp. BTA1.Item Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Biosfir Giam Siak Kecil-Bukit Batu Riau Terhadap Bakteri Dan Jamur(2017-09-11) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; FahrizawatiCagar Biosfir Giam Siak Kecil-Bukit (Cagar Biosfir GSK-BB) merupakan hutan gambut dataran rendah. Tanah gambut ini merupakan habitat utama mikroba tersterial salah satunya aktinomisetes. Aktinomisetes memiliki kemampuan menghasilkan berbagai metabolit sekunder. Oleh karena itu, dilakukan penelitian yang bertujuan untuk mengeksplorasi aktinomisetes asal tanah gambut Cagar Biosfir GSK-BB dan melakukan uji daya hambat aktinomisetes terhadap bakteri dan jamur. Sebanyak 33 isolat aktinomisetes yang diisolasi dari sampel tanah gambut menggunakan metode pour plate dalam medium Strach Casein Agar, 2 isolat (GSK.5.7 dan GSK.5.10) mampu menghambat S. pyogenes dan 1 isolat (GSK.4.9) mampu menghambat E. Coli, 2 Isolat (GSK.4.3 dan GSK.4.5) mampu menghambat pertumbuhan F. oxysporum, dan 11 isolat (GSK.1.1, GSK.4.1, GSK.4.4, GSK.4.6, GSK.4.7, GSK.4.8, GSK.4.10, GSK.4.12, GSK.5.5, GSK.5.6, dan GSK.5.9) mampu menghambat pertumbuhan Ganoderma sp. BTA1.Item Eksplorasi Dan Uji Daya Hambat Aktinomisetes Asal Tanah Gambut Cagar Biosfir Giam Siak Kecil-Bukit Batu,Riau Terhadap Jamur(2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Fahrizawati, FahrizawatiTanah merupakan habitat utama mikroba teresterial sekaligus sumber utama mikroba yang banyak digunakan dalam industri. Tiap-tiap tanah karena perbedaan sifat fisikokimianya menyebabkan pada masing-masing tempat memiliki kekhasan mikroba yang berbeda-beda. Crueger dan Crueger (1984), menjelaskan bahwa kelimpahan mikroba di dalam tanah bervariasi diantaranya genus Arthrobacter jumlah kelimpahan 5-60%, Bacillus 7-67%, Pseudomonas 3-15%, Agrobacterium 1-20%, Alcaligenes 2-12%, Flavobacterium 2-10% dan Actinomycetes 10-33%. Menurut Madigan, Martinko dan Parker (1994), aktinomisetes merupakan kelompok bakteri yang berbentuk filamen, Gram Positif dan membentuk cabang-cabang filamen (miselium) serta dapat hidup pada lingkungan yang beragam dan ekstrim. Aktinomisetes memiliki warna yang buram atau opak, tidak mengkilap, permukaan bertepung serta melekat kuat pada agar. Permukaan yang bertepung merupakan kumpulan hifa yang terdiri dari spora. Morfologi ini hanya terlihat pada aktinomisetes dewasa sedangkan pada koloni yang masih muda hanya terdiri dari hifa. Aktinomisetes dapat merubah warna medium karena memiliki warna yang mudah larut dalam air dan akan berdifusi ke dalam medium.Oskay, Tamer dan Azeri (2004) menjelaskan aktinomisetes mampu menghasilkan berbagai metabolit sekunder yang berbeda-beda seperti antibiotik, herbisida, pestisida, anti parasit dan enzim-enzim seperrti sellulosa dan xilanase. Dalam studi ini, sampel tanah akan dikoleksi dari berbagai habitat berbeda pada kawasan Cagar Biosfir Giam Siak Kecil-Bukit Batu Riau untuk dikaji keragaman aktinomisetesnya. Aktinomisetes yang diisolasi akan diuji daya hambatnya terhadap jamur.Item ISOLASI BAKTERI DAN JAMUR ENDOFIT DARI TANAMAN MANGGA LIAR MACANG (Mangifera foetida Lour)(Elfitra, 2023-04) Yannizur, R. Annisa; Roza, Rodesia MustikaMacang (Mangifera foetida Lour) belongs to the Anacadiaceae family which contains secondary metabolites such as flavonoids, tannins, phenols, saponoins, alkaloids and steroids which act as antibacterials. In general, the compound content in M. foetida is in line with the content produced by endophytic microbes in its plant tissues. This study aimed to isolate endophytic bacteria and endophytic fungi from the leaves and bark of the mango macang (M. foetida) plant. The study was conducted using the direct method. The results showed that 11 isolates of endophytic microbes were successfully obtained ie., five isolates of endophytic bacteria and six endophytic fungi isolateItem Isolasi Dan Aktivitas Antimikroba Aktinomisetes Asal Tanah Rizosfer Cagar Biosfer Giam Siak Kecil-Bukit Batu Riau(wahyu sari yeni, 2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, Atria; Haloho, LindasariThe aims of study are to isolate actinomycetes from rhizosphere soil, to determine the ability of actinomycetes to inhibit the growth of bacteria (Streptococcus pyogenes and Escherichia coli) and fungi (Fusarium oxysporum and Ganoderma BTA1). A total of 15 actinomycetes strains were recovered from rhizosphere soil samples using pour plate method with Starch Casein Agar (SCA). The results showed that 7 isolates (TBR1, TBR2, TBR3, MHGI1, MHG3, MHG4, FPR4) were active againts all microbial targets, 1 isolate (MHG2) was active againts S. pyogenes, F. oxysporum, and Ganoderma sp BTA1, 2 isolates (MHG5, FPR3) were active againts S. pyogenes, E. coli and F. oxysporum, and Ganoderma sp BTA1, 1 isolates (FPR1) was active againts S. pyogenes, E. coli and F. oxysporum, 1 isolate (FPR5) was only active againts Ganoderma sp BTA1, 2 isolates (TBR4, FPR2) were only active againts E. coli, and isolates (AKA1) was not active againts all microbial targets.Item Isolasi Dan Aktivitas Antimikroba Aktinomisetes Asal Tanah Rizosfer Cagar Biosfer Giam Siak Kecil-Bukit Batu Riau(2017-10-11) Roza, Rodesia Mustika; Linda, Tetty Marta; Martina, AtriaThe aims of study are to isolate actinomycetes from rhizosphere soil, to determine the ability of actinomycetes to inhibit the growth of bacteria (Streptococcus pyogenes and Escherichia coli) and fungi (Fusarium oxysporum and Ganoderma BTA1). A total of 15 actinomycetes strains were recovered from rhizosphere soil samples using pour plate method with Starch Casein Agar (SCA). The results showed that 7 isolates (TBR1, TBR2, TBR3, MHGI1, MHG3, MHG4, FPR4) were active againts all microbial targets, 1 isolate (MHG2) was active againts S. pyogenes, F. oxysporum, and Ganoderma sp BTA1, 2 isolates (MHG5, FPR3) were active againts S. pyogenes, E. coli and F. oxysporum, and Ganoderma sp BTA1, 1 isolates (FPR1) was active againts S. pyogenes, E. coli and F. oxysporum, 1 isolate (FPR5) was only active againts Ganoderma sp BTA1, 2 isolates (TBR4, FPR2) were only active againts E. coli, and isolates (AKA1) was not active againts all microbial targets.Item ISOLASI DAN KARAKTERISASI AKTINOMISETES RIZOSFER DARI TANAMAN SENTUL (Sandoricum koetjape (Burm.f.) Merr.)(Elfitra, 2021-12) Humairoh, Hanifah; Roza, Rodesia MustikaActinomycetes is a group of gram-positive bacteria that produce various secondary metabolites and generally live in the soil, especially in the rhizosphere of plants including Sandoricum koetjape. This study aimed to isolate and characterize rhizosphere actinomycetes from S. koetjape plants. Soil samples were collected from the plant rhizosphere, then diluted and inoculated by pour plate on SCA media. The actinomycetes isolates obtained were characterized. The results showed that, seven isolates of actinomycetes had circular and irregular colony shapes. The edges of the colonies were serrate, entire, and undulate. Colony elevation could be raised, umbonate, convex and flat. In addition, various color of the colonies were white, cream and gray.Item ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli(2014-03-28) Elfina, Dewi; Martina, Atria; Roza, Rodesia MustikaGarcinia mangostana Linn is one of the popular local plants that has been used as medicinal herb. Extract of Garcinia mangostana has been reported having anti-inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactive compounds from extract of mangosteen fruit husk were thought to produce by endophytic fungi. The purpose of this research were to isolate and characterize endophytic fungi from mangosteen fruit husk that potential to produce antimicrobial compound. Antimicrobial activity was determined by measuring the inhibition zone with Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candida albicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolates endophytic fungi which were obtained from mangosteen fruit husk were known to have antimicrobial activities. Endophytic fungi producing antimicrobial belongs to genus Aspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungi having the highest antimicrobial activities in agar disc assay were Penicillium sp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR and Aspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities in Kirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicillium sp.1KMA was significantly different with the other four endophytic fungiItem ISOLASI DAN KARAKTERISASI PARSIAL AKTINOMISETES DARI TANAH MANGROVE Avicennia alba Blume(perpustakaan UR, 2021-10) Kurniafebi, Fitri Aulia; Roza, Rodesia MustikaActinomycetes are gram-positive bacteria that have the ability to adapt in various environmental conditions. Mangrove forests are one of the ecosystems that have extreme environmental conditions. The purpose of this study was to explore and characterize activities of actinomycetes isolated from soil where Avicennia alba Blume population grown in Kuala Enok. Soil samples were taken from three different points. Isolation of actinomycetes using pour plate method with Starch Casein Agar (SCA) media. The results of isolation obtained in this study was four actinomycetes with varied morphology. Microscopic characteristics showed that the isolates are positive gram. In addition, biochemical tests showed negative results on catalase tests, while in amylase tests A4 isolates showed positive result.Item ISOLASI DAN SELEKSI BAKTERI ASAM LAKTAT DARI YOGHURT PRODUKSI INDUSTRI RUMAH TANGGA DI PEKANBARU YANG BERSIFAT ANTIBAKTERI TERHADAP Escherichia coli DAN Salmonella typhi(2014-03-28) Yani, Lili; Roza, Rodesia Mustika; Martina, AtriaThe occurance of Lactic Acid Bacteria (LAB) in yoghurt produced by home industry in Pekanbaru as probiotic potentially improve intestinal physiological function, useful for health as well as prevention of the growth of pathogenic bacteria. The purposes of this study were to calculate the total LAB population of yoghurt with the effect of mango and strawberry juice during different storage process, and also to determine the ability of antibacterial activity against Escherichia coli and Salmonella typhi. The population of LAB in yoghurt was 4,0 × 107 – 2,9 × 108 CFU/ml. Antimicrobial activity assay used the well diffusion method. The mango juice yoghurt incubated in 7 day was the most effective for Escherichia coli with the inhibition zone 27,1 mm. While the mango juice yoghurt incubated in 3 day was the most effective for Salmonella typhi with inhibition zone 29,9 mmItem Isolasi Dan Seleksi Bakteri Termotoleran Penghasil Xilanase Dari Kompos(wahyu sari yeni, 2018-08-23) Roza, Rodesia Mustika; Linda, Tetty Marta; Sihombing, IkaPenelitian ini bertujuan untuk menyeleksi dan mengkarakterisasi bakteri termotoleran penghasil xilanase dari kompos. Isolasi dan seleksi bakteri penghasil enzim xilanase dilakukan dengan menggunakan medium yang mengandung xilan 0,5 % diinkubasi selama 72 jam pada suhu 50oC. Bakteri yang mampu menghasilkan xilanase akan membentuk zona bening. Diperoleh sembilan isolat bakteri yang mampu menghasilkan xilanase. Aktivitas xilanase dihitung berdasarkan rasio zona bening per diameter koloni (Z/K). Berdasarkan rasio ini aktivitas xilanase yang paling tinggi diperoleh dari isolat KX7 dengan rasio Z/K 3,17. Isolat ini termasuk Gram positif, berbentuk batang, mampu membentuk spora, bereaksi positif terhadap uji katalase, uji oksidase, uji VP, uiji reduksi nitrat, uji karbohidrat (glukosa dan galaktosa).Item ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI AKTINOMISETES DARI ARBORETUM UNIVERSITAS RIAU(2017-01-10) Rahayu, Fatimah; Roza, Rodesia Mustika; Pratiwi, Nova WahyuDemand of antibiotics for phatogenic bacteria is getting increased, therefore, the discovery of novel antibiotics is needed to address antibiotics resistancy problem. Actinomycetes are well known to produce secondary metabolites such as antibiotics. Isolation of actinomycetes was conducted by making initial treatment of 4 soil samples with dry heat and wet heat to inoculate on Starch Casein Agar (SCA). From 4 sampled location at Univesitas Riau Arboretum, Location 3 had the most various of actinomycetes discovered. Wet heat treatment gave higher number of isolated actinomycetes with a number of 23 isolates. In this study, total actinomycetes isolated from samples were 30 isolates and tested their antibacterial activity. Actinomycetes isolate code C2.20 had the widest clear zone of 21.29 mm against pathogenic bacteria Staphylococcus aureus with agar disc method.Item ISOLASI FUNGI PENDEGRADASI HIDROKARBON MINYAK BUMI DARI SAMPEL TANAH TERCEMAR TUMPAHAN MINYAK BUMI(2016-01-28) Ningsih, Gustia; Martina, Atria; Roza, Rodesia MustikaBioremediation is a technique that can be used to solve the environment from oil spill. The purpose of this research is to isolate fungi from soil that contaminated with oil spill, and to test its ability in degrading crude oil. The ability of fungi to grow on the Bushnell Haas that containing crude oil was done by growing fungi in Bushnell Haas agar. The ability of fungi in degrading crude oil was tested with culturing it in Bushnell Haas broth that contain crude oil for 25 days. Twelve fungi isolates had been isolated from contaminated crude oil soil in Minas and Lirik. The growth test result indicated that isolate LL-A05 had the largest diameter colony (4.83 cm), while isolate LL-A06 had the smallest diameter colony (0.87 cm). All isolate have the ability in degrading crude oil. Isolate LL-A07 had the highest ability of degradation (86%) and isolate LL-A06 had the smallest degradation (69%).Item KARAKTERISASI PARSIAL AKTINOMISETES HASIL ISOLASI DARI RIZOSFIR TUMBUHAN SIAK-SIAK (Dianella ensifolia (L.) DC.)(perpustakaan UR, 2021-10) Rasmida, Hasnya Yuqa; Roza, Rodesia MustikaActinomycetes are gram-positive bacteria whose colonies are similar to fungi because they have hyphae. Actinomycetes have been known as strains that produce metabolites that play an important role in various fields. The potential of actinomycetes is high, so it is necessary to isolate and to characterize their properties. The bacteria were isolated from rhizosphere soils that have dense microbial population. The study successfully obtained two isolates from the rhizosphere soil of the siak-siak (Dianella ensifolia) plants. The two isolates showed different morphological characteristics and biochemical test results