Browsing by Author "Roza, R. M."

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    ISOLASI DAN SELEKSI JAMUR SELULOLITIK DARI HUTAN ARBORETUM UNIVERSITAS RIAU
    (2014-03-28) Hardianty, Denty Irma; Roza, R. M.; Martina, A.
    The Arboretum University of Riau constitutes of a secondary forest in Pekanbaru. Many plant genera exist in this Arboretum, therefore the litter produced has high content of cellulosa that may provide suitable environment for many cellulolitic fungus. The purpose of this study was to obtain the cellulolytic fungus isolates by screening the soil that collected from Arboretum University Riau, using Potato Dextrose Agar medium. The selection of cellulolytic fungus used Carboxyl Methyl Cellulose medium and was indicated by the appearance of clear zona. Cellulase activities were determinated using the ratio of clear zone and colony diameter. The result identified 53 cellulolytic fungus isolates. The L4H4 isolate had the highest ratio (3,51). There were three ratio criterions of clear zone to colony diameter in this study, i.e. high (24,53%), medium (35,85%) and low (39,62%). The characterization result showed that 15 fungus isolates belonged to the genera Aspergillus, Penicillium, Paecilomyces, Mucor and 2 isolates were unidentified
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    POTENSI MIKROBA INDIGENUS ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU DALAM MENGHAMBAT PERTUMBUHAN Xanthomonas oryzae pv. oryzae
    (2014-03-28) Wizra, N.; Roza, R. M.; Martina, A.
    The plant disease caused by Xanthomonas oryzae pv. oryzae is still the major problem that can reduce agriculture productivity up to 20%. Currently the use of chemical bactericides can cause environment pollution and resistance to bacteria. Microorganisms can be used as biocontrol agent that is environmental friendly and potential to be developed. This study aimed to find the potential microbe from peat land at Rimbo Panjang village, Kampar Riau to produce antibacterial activity against Xanthomonas oryzae pv.oryzae. Antibacterial activity of microbe was tested using agar disk method and measured based on the ratio between the clear zone and colony diameter (ratio Z/K). The results showed 21 isolates of actinomycetes and 23 fungus isolates had antibacterial activity. The highest ratio activity for actinomycetes isolates was produced by Streptomyses RBIS6 (2.98) while the highest ratio for fungus isolates was produced by of Penicillium sp. L5J15 (2.00). Actinomycetes were the most potential microbes to produce antibacteria than the other microbes.
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    SELEKSI MIKROBA SELULOLITIK DALAM MENDEGRADASI LIGNIN ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU
    (2014-03-28) Adlini, N. I; Fibriarti, B. L.; Roza, R. M.
    Peatland conversion activities into plantation can cause a low level of decomposing organisms growth, so the process of decomposition organic materials such as lignin and cellulose occurs imperfectly. Lignocellulolytic microbes such as bacteria, fungi, and actinomycetes may degrade lignin and cellulose to produce extracellular ligninase and cellulase enzyme. The purpose of this study to be obtain the lignocellulolytic microbial isolates by screening the cellulolytic bacteria, fungi, and actinomycetes isolates that may degrade lignin. The ability of a total of three bacteria isolates, 63 fungi isolates and 42 actinomycetes isolates isolated from peat soil at Rimbo Panjang have been known degrading cellulose but their ability to degrade lignin have not been known yet. Ligninase enzyme activities were determined by the ratio of clear zone and colony diameter. The result showed that three isolates of bacteria, 26 isolates of fungi and 40 isolates of actinomycetes had ligninolytic activity. The highest ratio of ligninolytic activity from bacteria was produced by L410-3a2 isolates (4,00), from fungi was produced by Penicillium sp. L5J1 (2,17) and from actinomycetes was produced by Frankia sp. L3A7 (12,29). The characterization result showed that 14 of lignocellulolytic fungal isolates were obtained from the genus of Penicillium, Trichoderma, Aspergillus and Rhizopus. The characterization result showed that 16 of lignocellulolytic actinomycetes isolates were obtained from the genus Streptomyces and Frankia while five isolates have not yet been identified
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    UJI AKTIVITAS LIGNINOLITIK JAMUR PEKTINOLITIK TERMOTOLERAN INDIGENUS RIAU
    (2014-03-28) Febriani; Martina, A.; Roza, R. M.
    This study was aim to test the ligninolytic activity of 19 thermotolerant pectinolytic fungal isolates, which are indigenous from Riau. The selection of ligninolytic isolates was done using agar disk method. Isolates which showed ligninolytic activity were quantitatively tested using a completely randomized design with two factors: the temperature and isolates. The treatments in the first factor were the room temperature and 50 oC. The second factor were 5 isolates that had high ratio activity. The result showed that the ligninolytic activity at 50 oC was greater than at room temperature. Penicillium sp. PNE4 had the highest ratio activity (12.07) at 50oC. The temperature and isolates significantly affected in decreasing Poly R-478 concentration. Trichoderma sp. PNE13 isolate had the highest activity in degrading lignin than the other isolates at room temperature (46.52%) and 50°C (47.92%). Temperature affected the weight of biomass significantly. The biomass at room temperature was higher than 50 oC. The highest biomass at room temperature was produced by Aspergillus sp.2 (0.555 g/L), while the highest biomass at 50 oC was produced by Penicillium sp. PNE4 (0.359 g/L)
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    UJI AKTIVITAS SELULASE JAMUR PEKTINOLITIK TERMOTOLERAN INDIGENUS RIAU
    (2014-03-28) Mira; Martina, A.; Roza, R. M.
    The indigenous isolates from Riau were known to have pectinolytic activity and thermotolerant, but their cellulase activity is still unknown. This study was aim to test the cellulase activity of 19 thermotolerant fungal isolates which are indigenous from Riau. The selection was done by using agar disk method. The determination of cellulase activity and biomass used a factorial complete randomized design (CRD) that consisted of two factors i.e. temperature and isolate. The result showed cellulase activity at 50°C was higher than at room temperature. Aspergillus fumigatus TT had the highest cellulolytic activity whereas Aspergilus sp.2 had the lowest cellulolytic activity. Temperature and isolates affected cellulase activity. Penicillium sp. PNE17 at 50°C had the highest cellulase activity and no significantly difference than Acremonium sp. PNE10. Temperature and isolates affected the biomass significantly but no interaction. Penicillium PNE17 had the highest biomass at room temperature (0,835 g/L) and at 50°C (0,330 g/L)