Browsing by Author "Rafi, Mohamad"

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    AKTIVITAS ANTIOKSIDAN FERMENTASI KO-KULTUR ISOLAT JAMUR LOKAL RIAU Penicillium sp. DAN Trichoderma sp.
    (Elfitra, 2022-07) Ferani, Aisya; Nurulita, Yuana; Rafi, Mohamad
    Local isolates of the Riau fungus Penicillium sp. LBKURCC34, Trichoderma asperellum LBKURCC1 and Trichoderma asperelloides LBKURCC2 are well-known potential producers of secondary metabolites with several bioactivities, such as antioxidants. This study aims to determine the antioxidant activity of the co-culture extracts of these local isolates of Riau. Production of the compounds by co–culture fermentation and extraction of their fermentation media, Penicillium sp. LBKURCC34 and Trichoderma asperellum LBKURCC1 (Co-Culture A), Penicillium sp. LBKURCC34 and Trichoderma asperelloides LBKURCC2 (Co-Culture B) and the three fungi (Co-Culture C) were performed using organic solvents butanol (BuOH), ethyl acetate (EtOAc) and acetone (Ace). The antioxidant activity was analyzed by DPPH. Based on the research, co-cultured extracts of Butanol C and Acetone C were the most active antioxidant activity with IC50 values of 48.428 ± 3.956 μg/mL and 43.331 ± 3.994 μg/mL respectively.
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    STUDI ANTAGONISTIK Trichoderma asperellum LBKURCC1 DAN Penicillium sp. LBKURCC34
    (Elfitra, 2023-12) Nasution, Ariq Fadhil Shidiqi; Nurulita, Yuana; Rafi, Mohamad
    Trichoderma asperellum LBKURCC1 and Penicillium sp. LBKURCC34 are a fungi isolated from plantation soil and peat soil in Riau Province. This study aims to examine the antagonistic activity between local isolates of Riau fungi; LBKURCC1 and LBKURCC34. The aim of the antagonistic test is to measure and determine the ability of antagonistic fungi to suppress the growth and development of pathogenic fungi or fungi tested in close proximity to the laboratory scale co-culture method. Antagonistic tests can be carried out in vitro using the dual culture method. Antagonistic tests are carried out for 14 days and observations are made in control and test petris. In the antagonistic test, the LBKURCC1 fungus showed stronger antagonistic inhibition than the LBKURCC34 fungus.