Browsing by Author "Pringgenies, Delianis"

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    Effect of Red Seaweed (Eucheuma cottonii) Powder Administration to The Quantity and Quality of Spermatozoa of Allethrin-Exposed House Mice (Mus Musculus)
    (2016-03-22) Pringgenies, Delianis; Ghofur, Abdul; Azizah, Ria; Ridlo, Ali
    Allethrin is an active chemical agent used in several variants of mosquito repellant products. When inhaled, allethrin is suspected to disrupt the quality of spermatozoa characterized by the reduction in amount, motility, and viability as well as the increase in morphological anomalies. This research is aimed to determine the effect of Eucheuma cottonii powder towards the quality and quantity of spermatozoa of house mice (Mus musculus) exposed by allethrin from electric mosquito repellant device. This research was carried out using laboratory experimental method with completely randomized design. The observation of the quality and quantity of spermatozoa were conducted right after acclimatization process (T0), at day 14 (T1), day 28 (T2), and day 42 (T3) after acclimatization process. Parameters of spermatozoa observed were the quantity, motility, morphology, and viability. Data were tested by One-way ANOVA and, if proven influential, were followed up by Tukey test information. The results showed that Eucheuma cottonii’s powder could only increase the quantity and motility spermatozoa of mice exposed to allethrin.
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    Potential of Sea Cucumber Rivet Red Extract (Holothuria leucospilota) As Antibacterial MDR (Multi Drug Resistant)
    (2016-03-30) Pringgenies, Delianis; Ridlo, Ali; Pratiwi, Henni
    Resistance of microorganisms to antibiotics is a serious problem in the treatment of infections of this period, so that necessity search for new bioactive compounds was inevitable. Secondary metabolites found in marine organisms is one of the alternative materials discovery of new antibiotics. The potential producers of bioactive compounds of secondary metabolite is sea cucumber. Rivet sea cucumbers Holothuria leucospilota is one of sea cucumbers found in abundance in Bandengan coast Jepara, but so far very limited use for marine pharmacology. The purpose of the study was to determine the antibacterial activity of the extract fractions rivet red sea cucumber (H. leucospilota) against MDR bacteria and compounds that have potential as antibacterial MDR. Analysis of samples of sea cucumbers includes extraction, fractionation, and analysis of bacterial sensitivity test Gas Chromatography-Mass Spectrometer (GC-MS), the extraction process is carried out by solid-liquid extraction method (solid-liquid). Fractionation is done with Open Column Chromatography (OCC). Test sensitivity of bacteria using the agar diffusion method according to the Kirby-Bauer. The results showed that the extract of Holothuria leucospilota has 7 fractions active against MDR bacteria Klebsiella sp and Enterobacter-10. The average value of inhibition zone is highest in fraction III with concentration of 80 ug / disk for each type of bacteria Klebsiella sp and Enterobacter 10 was 11.87 ± 0.90 mm and 11.49 ± 0.86 mm. The results of GC-MS analysis showed that the fraction IV containing the compound 2-methyl butanoic; 2-butoxy ethanol; 3,5,5 trimethyl 2-cyclohexane-1; propandiat phenyl and 2-methoxy-4-(2-propenyl) or eugenol.