Browsing by Author "Nugroho, Titania Tjandrawati"
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Item AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL 30% KULIT BUAH MANGGIS (Garcinia mangostana L.) SEBELUM DAN SESUDAH PROSES PENGERINGAN DALAM OVEN 60OC(2016-05-10) Simbolon, Lidya Purnama; Nugroho, Titania Tjandrawati; Teruna, Hilwan YudaEthanol extracts of mangosteen fruit rind contain high antioxidant activity that is heat labile. Drying of ethanol extracts containing antioxidant activity ideally is carried out at low temperatures using a vacuum freeze dryer. In the absence of a vacuum freeze dryer other methods must be used, such as oven drying but at temperatures with minimal loss of the antioxidant activity. To evaluate the effects of the drying process, the antioxidant activities of mangosteen fruit rind 30% ethanol extracts before and after oven drying at 60oC was compared to each other. Dried powdered mangosteen fruit rind was incubated in 30% ethanol for 8 days at 40ºC and 100 rpm in a shaking incubator. After incubation, the mixture was filtered to separate the ethanol extract from the remaining solids. The ethanol solvent from the filtered extract was evaporated by rotary vacuum evaporation at 50oC, and the semi-dried residue was further oven dried at 60oC to constant weight. The antioxidant activity of the semi-dried extract residue and oven dried extract was analyzed using the 2,2 diphenyl-1-picrylhydrazyl (DPPH) method. The results showed that the IC50 value of the antioxidant activity from mangosteen fruit rind 30% ethanol extract before the drying process was (42.78±1.54) μg/mL, and after the drying process was (21.30±2.29) μg/mL. Unpaired student t analysis to compare the average IC50 values of the 30% extracts before and after drying showed that there was a significant increase (p<0,05) in the antioxidant activity after the 60oC oven drying process.Item BIODEGRADASI POPOK BAYI MENGGUNAKAN JAMUR SELULOLITIK Trichoderma asperellum T.N.J63 DENGAN FERMENTASI PADAT(2016-05-10) Damayanti, Nelvi; Nugroho, Titania Tjandrawati; Haryani, YuliBaby diaper contents cellulose pulp that can be used as substrat in cellulose production. The objective of this study was to investigate baby diaper biodegradation abilities of cellulolytic fungi Trichoderma asperellum T.N.J63 which isolated from orange farmland in Riau province. Biodegradation process by solid fermentation was carried out for 10, 15, 20, 25, and 30 days. Parameters examined were activity of crude extract cellulose using Nelson-Somogyi method, organic carbon (TOC) using gravimetric method, nitrogen total (N-total) using Kjedahl method, and C/N ratio. The result showed that baby diaper can be useed as substrat to produce cellulose. The highest activity of crude extract cellulose was found at 25 days fermentation by T.N.J63 LBKURCC2 (5,78± 0,37) × 10-3U/mL. Total organic carbon was decreased during 30 days fermentation (29.668%), N-total during 30 days fermentation (0.418%), and C/N ratio was found to be 106.351.Item Biokimia Dan Potensi Bioteknologi Fungi Biokontrol Untuk Aplikasi Dalam Bidang Pertanian, Industri "Hijau" Dan Farmasi(2015-01-21) Nugroho, Titania TjandrawatiUntuk meminimalkan pemakaian pestisida kimiawi sintetik yang sering berdampak buruk bagi lingkungan dan kesehatan, sejak beberapa tahun telah dikembangkan fiangi biokontrol untuk perlindungan tarsman dari hama dan penyakit. Fungi biokontrol adalah fungi, atau yang lebih umum dikenal sebagai jamur benang, yang dapat menghambat secara biologis pertumbuhan patogen tanaman, parasit atau insekta. Terdapat beberapa kriteriayang harus dipenuhi oleh flingi untuk dapat digunakan sebagai fungi biokontrol, yaitu fiingi tersebut tidak bersifat patogen terhadap hewan atau tanaman, kompatibel atau cocok dengan lingkungan pertumbuhan tanam^an, dan jika akan digunakan di lahan pertanian yang telah pemah dilakukan penyemprotan dengan pestisida sintetik, maka fungi biokontrol tersebut hams resistan terhadap residu pestisida yang tersisa.Item KADAR TOTAL FENOLIK EKSTRAK ETANOL 40% KULIT BUAH MANGGIS SEBELUM DAN SESUDAH PROSES PENGERINGAN OVEN 60ºC(2016-05-02) Maula, Nikmattul; Nugroho, Titania Tjandrawati; Teruna, Hilwan YudaThe total phenolic content of mangosteen fruit rind 40% ethanol extract before and after drying process was analyzed using the Folin-Ciocalteau method. For the extraction process, powdered mangosteen fruit rind was incubated with 40% ethanol for 8 days at 40ºC in a 100 rpm shaking incubator. The extract was separated from the remaining solids by paper filtration, and the filtrate was evaporated using a vacuum rotary evaporator at 50oC. The partially dried extract obtained after rotary evaporation, was oven dried at 60ºC until constant weight. The results showed that total phenolic content from extract ethanol 40% of mangosteen fruit rind before and after drying process was (13.51±4.96) mg GAE/g extract and (24.88±7) mg GAE/g extract respectively. Student t analysis to compare the average total phenolic content of the two treatments showed that there was no significant difference of total phenolic content before and after the drying process (p≥0.05). From this study, it can be concluded that there is no detectable loss of total phenolic compounds caused by the drying process at maximum temperatures of 60oC.Item PENENTUAN TOTAL KONSENTRASI ANTOSIANIN DARI UBI JALAR UNGU (Ipomoea batatas L.) DENGAN METODE pH DIFERENSIAL SPEKTROFOTOMETRI(2016-05-02) Octaviani, Dinda Yulia; Nugroho, Titania Tjandrawati; Dahliaty, AndiPurple sweet potato (Ipomoea batatas L.) is potential as a natural antioxidant based on the high concentration of anthocyanins contained therein. The purpose of this study was to determine the total concentration of anthocyanins from purple sweet potato experiencing incubation buffer acetate using spectrophotometric differential pH method. Samples were incubated in acetate buffer at 40oC for 60 hours at a speed of 170 rpm. The process is continued by adding methanol to 70% that has been acidified with HCl 1% so that the solvent to be pH 3.5. Extracts were obtained and diluted in pH 1 and pH 4.5 solvents and then the concentration of anthocyanin determined based by the spectrophotometric differential pH method. By this method it could be determined that the total anthocyanin concentration extracted was (120.64±44.234) mg/100 g.Item PENGARUH ANTIBIOTIK AMOXICILLIN TERHADAP PERTUMBUHAN DAN AKTIVITAS ENZIM LAKASE JAMUR Trametes hirsuta D7(Elfitra, 2023-05) Hababil, Andini Rulia; Nugroho, Titania Tjandrawati; Anita, Sita HerisAmoxicillin is a β-lactam antibiotic derived from aminopenicillin which is bactericidal and inhibits bacterial cell wall synthesis. The existence of amoxicillin that spread in nature needs to be researched whether its presence in nature can interfere the growth of biological plants, one of which in this study is fungi. This study aims to examine the effect of the antibiotic amoxicillin on the growth of white-rot fungi Trametes hirsuta D7 and its laccase activity. The results indicated that the addition of amoxicillin had no effect on the growth rate of Trametes hirsuta D7. The addition of amoxicillin at concentrations of 100 and 300 ppm resulted in a maximum growth rate of 1.48 ± 0.05 cm/day. Furthermore, the maximum biomass and laccase activity were 0.221 ± 0.015 g and 0.025 ± 0.006 U/mL, respectively. This study shows that Trametes hirsuta D7 have the potential to be used to degrade amoxicillin.Item PENGARUH BERBAGAI pH TERHADAP SPEKTRUM ZAT WARNA TEKSTIL REACTIVE BLACK 5(Elfitra, 2023-01) Nabilah, Putri; Nugroho, Titania Tjandrawati; Nurulita, YuanaReactive Black 5 (RB5) dyes are a group of azo dyes that are widely used for dyeing fabrics. The purpose of this study was to determine the effect of the pH used in different variations, namely at pH 4,5, pH 5,5 and pH 6,5 on the resulting RB5 color spectrum, as measured by its absorbance of visible light. Based on this study, there were no significant differences observed in the visible light absorbance maximum wavelengths of RB5 at different pHs. The RB5 visible light absorbance spectrum resulted in a broad peak, with an approximate absorbance maximum at 600 nm at pH 4,5, pH 5,5 and pH 6.5. Although not causing any shift in the maximum absorbance wavelength, there was a difference in the resulting absorbance intensity at different pH. So, it can be concluded that the use of pH variations between pH 4,5 to pH 6,5 can cause differences in absorbance intensity, but no shift in RB5 maximum absorbance wavelength.Item PRODUCTION OF AN ANTIBACTERIAL COMPOUND AGAINST THE PLANT PATHOGEN Erwinia carotovora subs. Carotovora BY THE BIOCONTROL STRAIN Gliocladium sp. T.N.C73(2014-05-13) Saputra, Hendra; Puspita, Fifi; Nugroho, Titania TjandrawatiGliocladium sp. T.N.C73 was originally isolated as a biocontrol agent from suppressive soil against the phytopathogen Phytophtora capsici. Apart from producing chitinase, this biocontrol fungal strain also produces secondary metabolites having antibacterial properties against the Gram positive bacteria Bacillus subtilis and Staphylococcus aeureus. In order to further explore the ability of Gliocladium sp. T.N.C73 in managing plant diseases caused by bacteria, ethyl acetate extracts of the fermentation medium of this strain was analyzed for its ability to inhibit the Gram negative Erwinia carotovora subsp. carotovora that causes soft rot disease in food crops. Disc diffusion bioassays showed that the extracts could inhibit E. carotovora subsp. carotovora growth. Depending on the initial spore concentration of the fermentation medium inoculum, several compounds were detected by thin layer chromatography (TLC) of the ethyl acetate extracts that fluorescence under UV light, and one compound that gave a bright red spot when sprayed with a p-anisaldehyde solution.Item PRODUKSI ENZIM β-GALAKTOSIDASE DARI JAMUR ENDOFIT Fusarium oxysporum LBKURCC41 YANG DIISOLASI DARI UMBI DAHLIA(2016-05-10) Rusli; Nugroho, Titania Tjandrawati; Saryonoβ-Galactosidase is an enzyme that can catalyze the lactose hydrolysis reaction into glucose and galactose. This study was carried out to determine the ability of endophyte fungi Fusarium oxysporum LBKURCC41 to produce β-galactosidase enzyme. This fungi was grown in production media : lactose 10 g, yeast extract 20 g, MgSO4 1 g and K2HPO4 1 g per liter destilat water. The β-galactosidase activity of the enzyme was determined based on the amount of oNP (o-nitrophenol) liberated in Unit/mL from the substrat oNPG (o-nitrofenil-β-D-galaktopiranosida), while the specific activity of the enzyme was indicated by activity per unit weight of proteins. The protein concentration of enzyme extracts was determined by the Lowry method. The results showed that the fungi tasted was able to produce enzyme β-galactosidase. Enzyme activity of isolate LBKURCC41 at 2, 4, 6, 8, and 10 days of production time were 0.146 ± 0.060 Unit/mL, 0.150 ± 0.058 Unit/mL, 0.134 ± 0.040 Unit/mL, 0.137 ± 0.037, and 0.132 ± 0.075 Unit/mL respectively. No real difference activity for each of production time base on ANOVA α≥0,05. Specific activity of the isolate was 1.568 ± 0.140 Unit/mg protein.