Browsing by Author "Nugroho, Titania Tj."

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    AMPLIFIKASI GEN CaM Penicillium sp. LBKURCC34 SECARA POLYMERASE CHAIN REACTION
    (Elfitra, 2022-06) Risdarenuadie, Septiani; Nugroho, Titania Tj.; Nurulita, Yuana
    The fungal isolate Penicillium sp. LBKURCC34 is one of the isolates that has been successfully isolated from peat soil in the core zone of the Giam Siak Kecil-Bukit Batu Biosphere Reserve (GSKBB), Riau Province. Fungal isolates have the potential to produce secondary metabolites with antimicrobial activity, and it is important to correctly know the species of the isolate for inventory reasons, ease of secondary metabolite isolation, purification and utilization. In this study, as a first step toward the molecular identification of Penicillium sp. LBKURCC34, the fungal chromosomal DNA was amplified in the CaM region using the Polymerase Chain Reaction (PCR) method. A horizontal electrophoresis process was carried out to detect the success of the amplification process and calculate the molecular weight or length of the DNA products. A PCR product corresponding to CaM amplified region was successfully obtained after 35 cycles with the following conditions: 45 seconds of template denaturation at 94oC, 45 seconds of primer annealing at 55oC, and 1 minute of primer elongation at 72oC. The final products were subjected to a final elongation for 10 minutes at 72o C. The PCR product of the CaM amplified region of Penicillium sp. LBKURCC34 had a DNA length of 785 bps.
  • No Thumbnail Available
    Item
    IDENTIFIKASI MOLEKULER JAMUR TERMOFILIK Aspergillus sp. LBKURCC304 ISOLAT LOKAL BUKIK GADANG SUMATERA BARAT
    (Elfitra, 2022-06) Nst, Fida Selfiana; Saryono, Saryono; Nugroho, Titania Tj.
    Fungi strain LBKURCC304 is a thermophilic fungi isolated from Bukik Gadang hot spring, West Sumatera, can produce amylase enzyme based on semi-quantitatively and quantitatively analysis. Morphological characteristics indicated that strain LBKURCC304 was a member of Aspergillus genera. Morphological identification cannot be used to determine species of fungi, therefore molecular identification is required. Determination of species is very important to explore the potential of LBKURCC304 with reference from the same species. Molecular identification of Aspergillus sp. LBKURCC304 was initiated by isolation of genomic DNA from 2 days old mycelia, PCR amplification on ITS-1 and ITS-2 rDNA regions were successfully performed using ITS4 and ITS5 primers at annealing temperatures of 45ºC. The sequences were verified with BioEdit program and then aligned to get the complete sequences. DNA sequences of Aspergillus sp. LBKURCC304 were analyzed using BLAST. The genomic DNA and PCR product have molecular weights of 16.425 bp and 593 bp, respectively. The BLAST analysis showed that Aspergillus sp. LBKURCC304 is Aspergillus fumigatus.
  • No Thumbnail Available
    Item
    IDENTIFIKASI MOLEKULER JAMUR TERMOFILIK Aspergillus sp. LBKURCC304 ISOLAT LOKAL BUKIK GADANG SUMATERA BARAT
    (Elfitra, 2022-06) Nst, Fida Selfiana; Saryono, Saryono; Nugroho, Titania Tj.
    Fungi strain LBKURCC304 is a thermophilic fungi isolated from Bukik Gadang hot spring, West Sumatera, can produce amylase enzyme based on semi-quantitatively and quantitatively analysis. Morphological characteristics indicated that strain LBKURCC304 was a member of Aspergillus genera. Morphological identification cannot be used to determine species of fungi, therefore molecular identification is required. Determination of species is very important to explore the potential of LBKURCC304 with reference from the same species. Molecular identification of Aspergillus sp. LBKURCC304 was initiated by isolation of genomic DNA from 2 days old mycelia, PCR amplification on ITS-1 and ITS-2 rDNA regions were successfully performed using ITS4 and ITS5 primers at annealing temperatures of 45ºC. The sequences were verified with BioEdit program and then aligned to get the complete sequences. DNA sequences of Aspergillus sp. LBKURCC304 were analyzed using BLAST. The genomic DNA and PCR product have molecular weights of 16.425 bp and 593 bp, respectively. The BLAST analysis showed that Aspergillus sp. LBKURCC304 is Aspergillus fumigatus.