Browsing by Author "Nugroho, T. T"

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    ISOLASI, UJI AKTIVITAS, DAN AKTIVITAS SPESIFIK ENZIM SELULASE Penicillium sp. LBKURCC27 SEMIMURNI MELALUI PENGENDAPAN (NH 4 ) 2SO 4
    (2014-03-27) Sarip, M.; Nugroho, T. T; Teruna, H. Y.
    Enzyme activity of cellulase can be concentrated by ammonium sulfate ((NH 4)2SO4) precipitation. The concentration was carried out by adding ((NH4)2SO4) 80% saturated precipitation. The precipitated enzyme was redisolved in 1/70 of the original crude extract volume. Enzyme activity of cellulase was assayed using CMC and avicel as the substrate. The results showed that in the concentrated enzyme preparation, the CMCase was increased 12 fold and enzyme activity of avicelase increased 3 fold. Activity and specific activity of the final CMCase preparation were 1.1160±0,0622 U/mL and 7.43±0,417 U/mg, respectively. Activity and specific activity of avicelase preparation were 0.0520±0.0735 U/mL and 0.34±0.487 U/mg, respectively.
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    METODE ISOLASI FUNGI PENGHASIL KITINASE DARI SAMPEL TANAH
    (2014-03-27) Uliya, R.; Nugroho, T. T; Dahliaty, A.
    Chitinase producing fungi are potential biocontrol organisms for plant protection. This is due to the ability of chitinase to degrade the plant pathogenic fungi cell walls that contain chitin. Many soil samples contain biocontrol fungi. To obtain fungal isolates that produce chitinase from a soil sample, selective medium containing chitin as the sole carbon source should be used. This short paper describes a method to isolate chitinase producing fungi using a selective media. Using this simple method, three chitinase producing fungal strains could be isolated from a secondary forest located at the Giam Siak Kecil Bukit Batu biosphere reserve.