Browsing by Author "Minarni"
Now showing 1 - 20 of 21
Results Per Page
Sort Options
Item ANALISA HASIL POLA FRINJI DENGAN KUALITAS CERMIN DATAR MENGGUNAKAN METODE KONTAK LANGSUNG DAN INTERFEROMETER MICHELSON(2016-02-01) Siregar, Abdul Hadi; Minarni; Emrinaldi, TengkuOptical components are found in everyday life like mirrors and lenses.For different types of measurement and research optical components characteristics of are very vital, quality of mirror such as surface quality and flatness are very important. In this research there are two systems were built to analyze the correlation between fringes and the quality of the mirrors, using the method of direct contact and Michelson interferometer method. The direct contact method was constructed using a white LED, an optical flat as the reference mirror, and 3 MP CMOS camera. The Michelson interferometer method was constructed using a diode laser source with the wavelength of 650 nm,where one of it’s mirror was an optical flat as a reference. The samples used were 3 mirrors with a 1/10 ⋋ precision, 4 mirrors with a 5 ⋋precision, and 2 mirrors with 4-6⋋ precison. The fringes were recorded with CMOS camera and the data was then processed using image processing software by measuring the fringe distance in the form of Pixel. The mirror with 1/10 ⋋ precision shows that the amount of fringes are greater and straighter with the average fringe amount of 8 fringes, the fringes from the mirror with the precision 5 ⋋the average fringes amount are 5 fringes and the total average fringes with precisionof 4-6 ⋋ are 7 fringes. The results showed that at contact method, 1/10⋋ mirrors had lesser fringi number than 5⋋ mirrors i.e 4 and 5 respectively and under fringe distancethan 5⋋mirrors which were 120 pixel and 161,3 pixel. The result showed for interferometer method, the fringe numbers were for 1/10 ⋋ than for 5⋋,and 4-6⋋ which were7,5, and 6 respectively while the fringe distances were smaller for 1/10⋋ mirror than 5⋋and 4-6⋋ mirrorItem ANALISA PENGARUH KADAR AIR TERHADAP FLUORESENSI KLOROFIL DAUN BAYAM MENGGUNAKAN METODE PENCITRAAN FLUORESENSI (FLUORESCENCE IMAGING)(2016-02-01) Ekayani, Fernika; Minarni; ZulkarnainFluorescence imaging is a method used to detect the early effects of environmental conditions on plants before the greater damage on plants happen. A fluorescence imaging optical system was built in this research consisted of two LEDs with different wavelength i.e. 450 nm and 680 nm, 3 MP CMOS camera and lens, and ND filters (neutral density filter). The intensities of LED light were varied using three different ND filters which were represented by their optical power. The samples were Amaranthus tricolor L. Spinach varitas. The plants were five different water volume i.e. 75 ml, 100 ml, 125 ml, 150 ml,and 175 ml. The differences between chlorophyll fluorescence intensity of the spinach leaves for all treatments were investigated. The images of leaves after illuminated by the LED lights were recorded by the CMOS camera. The fluorescenced intensities were found from RGB plot using ImageJ software. The research results show that the maximum fluorescence intensity spinach which grow with water volume 75 ml and the minimum fluorescence intensity spinach which grow with water volume 125 ml used LED with wavelength 450 nm and 680 nm. The difference of fluorescence intensity spinach which grow with water volume 75 ml and 125 ml used LED at wavelength 680 nm was seen higher when LED intensity was the lowest which was about 45,32 % difference.Item ANALISA PENGARUH SUDUT PENYINARAN TERHADAP GRAY VALUE PADA POLA SPEKEL BUAH APEL MENGGUNAKAN METODE LASER SPECKLE IMAGING(2016-02-01) Siregar, Rasmiana Poja; Minarni; Emrinaldi, TengkuSpeckle patterns are formed from scattered coherent lights which interfere each other when an object surface is illuminated by a coherent light. Speckles formed on the surface of biological object are called biospeckle. Laser Speckle Imaging (LSI) is a non-destructive imaging technique that can be used to detect the optical signal from biological tissue. A simple LSI system is consist of three main components, which are a laser as a light source, a sample, and a light detector. In this study, LSI system has been constructed using He-Ne laser as a light source, Fuji apples and Washington apples (Red Delicious) as samples and CMOS camera as detector. Angles of light incident on the samples were varied that was 30o, 45o, and 60o angles in order to know the optimum angle that gave the highest speckle patterns intensity. The speckle patterns represented by lever of gray value were analyzed using Image-J software. The result showed that the level of maximum gray value depended on the angle of illumination. The bigger the irradiation angle was the smaller the maximum of gray value. The maximum gray values Intensity Fuji apples were higher than Washington apples for each angle. The level of gray value maximum of speckle pattern apples at an angle of 30o consecutive were 127,6523 a.u for Fuji apples and 99,9951 a.u for Washington apples, at an angle of 45o were 111,0635 a.u for Fuji apples and 83,5215 a.u for Washington apples and at an angle of 60o were 102,8457 a.u for Fuji apples and 66,9639 a.u for Washington apples. That was because the surface of Fuji apples more roughers than Washington apples.Item ANALISA PEREKAMAN DAN REKONSTRUKSI HOLOGRAFI DIGITAL MIKROSKOPIK PADA KACA PREPARAT MENGGUNAKAN METODE IN-LINE(2016-02-01) Hasibuan, Marlan; Minarni; ZulkarnainiThis research applied in-line holography method and digital technique to record and reconstruct hologram. This method was used to create the holograms from two microscopic objects. The recording was done using a laser with a wavelength of 650 nm. The samples in microscopic slide used were cross section of hibiscus flower stem and leaves of citrus, CMOS camera was used as image recorder. The laser power was varied using 5 ND filters with it’s power of 3,6 mW, 2,24 mW, 1,2 mW, 0,36mW 0,047 mW and 0,005 mW and also without ND filter. The distance between beam expander and samples was varied and the magnification of objective lens used were also varied at magnification level of 10x, 40x, and 100x. The results of recording were reconstructed using holovision software and were then analyzed using ImageJ to find the grayscale average value. The final results of this research showed that the higher power of laser resulted in holograms with better clarity by the percentage difference between the highest and the lowest grayscale average value of 75 %. The distance between beam expander and samples influenced the intensity on samples, and the closer the lens to the samples, the higher the intensity on samples resulted in holograms with better clarity. Lens with magnification of 100x produced hologram with the best clarity over all the lens by 28 % of the percentage difference of grayscale average value.Item ANALISA POINTING STABILITY SINAR LASER MENGGUNAKAN QUADRANT PHOTODIODE (QPD)(2016-02-01) Azmi, Fauzul; Minarni; ZulkarnainiMeasurement of pointing stability level of some laser beams has been conducted. The measurement system consists of two different laser types, QPD, QPD amplifier circuit, digital oscilloscope, CCD Camera and image processing software. There ware two measurement quantities that were observed, the stability of beam position on QPD, and the stability of beam size on CCD camera. QPD output was amplifield by QPD amplifield circuit then displayed on digital oscilloscope. The laser beam was pointed to CCD camera. There were three lasers used, one is He-Ne laser 638 nm in wavelenght, two diode laser with 650 nm wavelenght but diffrent in brand. The results showed that the position of beam on QPD representated by frequency fluctuation on the QPD output and the beam size on the CCD camera changed over time. The average position displacement of the Aixiz laser was 0.0048 Hz and it’s diameter displacement was 0.062 mm. Average position displacement of the Roithner’s laser was 0.0016 Hz and it’s diameter displacement was 0.15 mm and He-Ne’s laser, average positon displacement was 0.003 Hz and it’s diameter displacement was 0.288 mm. The value of displacement level of position and diameter of a laser indicated their pointing stability level. So the He-Ne laser unsstable in position stability while Aixiz laser was more stable in diameter . It could be because it’s size bigger than otherItem ANALISA POLA DIFRAKSI FRAUNHOFER PADA CELAH TUNGGAL DAN PEMBUKTIAN PRINSIP KETIDAKPASTIAN HEISENBERG(2013-07-25) Alfa, R.; Minarni; SalomoA study on Fraunhofer diffraction pattern of a single slit using a He-Ne Laser and Heisenberg uncertainty principle has been conducted. A slit width (s) and a distance from slit to screen (L) are varied to investigate the resulted diffraction patterns. The slit width sizes used are 0,03 mm and 0,06 mm, and L varied from 300-1000 mm. Thedistance between two dark fringes are measured (∆w) until the 5th order and averaged. The an uncertainty constant for Heisenberg uncertainty (A) principle is calculated. The results showed that ∆w values increased as L larger. Uncertainty constant (A) is larger when the slit width (s) smaller with A values equal or bigger than 1, it means the Heisenberg uncertainty principle is proved for the two slits.Item ANALISA TINGKAT KEMASAKAN (RIPENESS) BUAH SAWO MENGGUNAKAN METODE PENCITRAAN FLUORESENSI(2016-02-01) Guciano, Ruli; Minarni; ZulkarnainFluorescence imaging and fluoresence spectrocopy are methods that can be used to detect the Ripeness level of sapodilla fruit (sawo fruit) based on the maximum fluorescence intensity. The development of efficient and economical fluorescence imaging system is needed to analyze the post-harvest ripeness of sapodilla. In this research, fluorescence imaging system was built using LED 680 nm as the laser source and CMOS 3 MP camera as a detector. LED was used to induce fluorescence on the sapodilla fruits. The samples used were Sapodilla Manila (Manilkara zapota (L.) van Royen) with two measurement variations. The first measurement was done on sapodilla with 3 levels of ripeness, those were ripe, half ripe, and unripe. The second measurement was done on the half ripe fruit for 6 day period. The image of fruit that was illuminated by LED light was recorded using the CMOS camera. Fluorescence intensity was presented in RGB plot using Image-j software. The relation between fruit ripeness level and the maximum fluorescence intensity was analyzed. The results of research on the first methods showed that the average fluorescence intensity of ripe sapodilla was higher than those with half ripe and unripe sapodilla fruits. The maximum fluorescence intensity indicated a different level of ripeness. The difference of fluorescence intensity between ripe and half ripe sapodilla is 7,8%, the difference of fluorescence intensity between ripe and unripe sapodilla is 35,9 %, and half ripe sapodilla had a difference of 30,9% with the unripe sapodilla. The results of the second method showed that at the fourth day, sapodilla was already in the process of rotting. On the second day to the fourth day, the fluoresecence intensity has increased. The fifth day and the sixth day, the fluorescence intensity has decreased linearly.Item ANALISIS HUBUNGAN KEKERABATAN BEBERAPA JENIS MANGGA (Mangifera) BERDASARKAN KARAKTER MORFOLOGI DAN FLUORESENSI KLOROFIL(2013-07-04) Swita, Anggi; Fitmawati; MinarniThis study aimed to characterizeand to determine mango species of Mangiferabased onthe morphological character and laser induced fluoresence of chlorophyll. The study had been conducted from December 2012 to March 2013 on 4 study sites in Riau Province by using a survey method anda direct observationto the morphological characters and a simple method to the fluoresence of chlorophyll. Data of morphological observation and fluoresence of chlorophyll was described in a character tabel. A total of 80morphological characters and fluoresence of chlorophyll were scored and analyzed using PAUP 4.0. 9 distinct mango species from Mangifera genus were determinedbased on the morphological characters and fluoresence of chlorophyll. The cladogram formed two main clads, clad I only consisted of M. torquendra and clad II consisted of M. foetida, M. odorata, M. indica, M. laurina, M. sumatrana, M. zeylenica, M. quadrifida and Mangifera sp.. All of the Mangiferaspecies in clad II were grouped with bootstrap value at 51%. The clustering result in the cladogram was mainly based on the similarity of morphological characters and the similarity of fluoresence colors.Item APLIKASI METODE FLUORESCENCE IMAGING UNTUK ANALISA KERUSAKAN MEKANIK REKAYASA PADA BUAH TOMAT(2016-02-01) Ningrum, Asdilita Okta Guma; Minarni; Emrinaldi, TengkuIdentification of abnormalities and damages on tomato fruits in post harvest is still conducted manually by observing their physical appearance. This research used fluorescence imaging to identify damages on some tomatoes which were treated intentionaly has been done. The tomatoes were bruished by hand pressure and soaked in hot boiling water. Fresh tomatoes without treatment were used as comparison. The fluorescence imaging system consisted of some optical components such as a lens, a filter, a 3mp CCD camera. There were two diode lasers with different wavelengths i. e 650nm and 532nm. The fluorescence intensities of the tomatoes after shoned by the laser light were measured from the RGB plot by Image-J software of images captured by the CCD camera. The results showed that the fluorescence intensities obtained from a red laser diode of the samples without treatment, the samples bruished by hand pressure, and the samples were soaked in hot boiling water were 72.3 a.u, 52.1 a.u, and 67.1 a.u. For the green laser diode with tomatoes without treatment, the sample bruished by hand pressure, and the samples soaked in hot boiling water were 93.9 a.u, 133.4 a.u, and 94.8 a.u. The tomatoes bruished by hand pressure has the highest the lowest when fluorescent intensity when induced by green laser diodes, and induced by red laser diodesItem BUILDING AN OPTICAL TWEEZERS FOR LIFE SCIENCE RESEARCHES(2014-05-21) MinarniOptical Tweezers has evolved as a valuable tool to trap and manipulate particles. Since it was introduced in 1986 by Ashkin and colleagues, Optical Tweezers has been used in many research areas. Its applications are found on research in atomic physics, medical physics, chemistry, and more notably in biophysics and cell biology. The simplest optical tweezers can be built from a commercial optical microscope either upright or inverted. The tweezing force comes from the radiation pressure of laser light which is sent to the back of the objective lens then is focused by the lens to the particles in a cuvette or slides. The particles can be any things from atoms, molecules, bacteria, viruses, blood cells to DNA. The optical tweezers are very useful in studying biological samples because it can provide picoNewton and nanometer ranges of force hence one can study the motor properties of the particles without damaging them. There are many parameters can describe a reliable optical tweezers. These parameters depend on the laser wavelength used and the size of the particles. In this article, the process to build an optical tweezers and what optical components needed will be described. The optical tweezers described has been used to trap two size-polystyrene beads with 830 nm laser light from a diode laser. This optical tweezers will be prepared to trap and study chlorophylls of mango leaves in searching for the new method in identifying its diversity. In the future, a portable and affordable optical tweezers for life science researches will be builtItem DESAIN SISTEM PENDETEKSI POSISI VERTIKAL DAN HORIZONTAL SINAR LASER MENGGUNAKAN QUADRANT PHOTODETECTOR (QPD)(2014-03-26) Yunus, M.; Minarni; Setiadi, R. N.A detection system of vertical and horizontal position using a Quadrant Photodetector (QPD) has been designed and made. The system consists of four level, i.e a QPD, an integrator circuit as current to voltage converter, summing and divider circuits, and a circuit of signal output X and Y. A quadrant photodetector is placed on a translation stage than can be arranged vertically (y) and horizontally (x) in a interval of millimeter. Positions of the laser beam are varied vertically and horizontally on the QPD so that output voltage of each segment of the QPD, integrator and output voltage X and Y can be observed. The positions of X and Y measured are plotted to x and y that are varied manually. The relation indicated a linear. For all segment of QPD the bigger segment of QPD that is irradiated by the laser beam then the larger output voltage is generated. The values of output voltage and will decrease when x position increasingly towards the negative, while the values of output voltage and will be increased. This is due to the laser beam diameter on the surface of A and B it gets smaller while on the surface C and D it gets larger when the x position is changed manually from the position -1,5 mm x 1,5 mm. The position of y is changed manually from the position -1,5 mm y 1,5 mm then the values of output voltage and will decrease when y position increasingly towards the negative, while the value an output voltage and will increase because the laser beam on surface of C and B it gets larger while on the surface A and D ist gets smallerItem KOREKSI BENTUK BERKAS CAHAYA LASER DIODA MENGGUNAKAN SEPASANG PRISMA ANAMORPIK(2014-07-01) Minarni; Iwantono; Pranata, Age. Laser diode adalah laser semikonduktor yang digunakan untuk berbagai aplikasi saat ini karena harga yang terjangkau, tersedia dengan berbagai panjang gelombang dan daya, mempunyai lebar bandwidth yang sempit, energi efisien, dan kompak.Namun laser diode mempunyai beberapa kelemahan yaitu berkas cahanyanya yang tidak simetris atau eliptikal, rentan terhadap fluktuasi arus injeksi dan suhu. Pada penelitian ini bentuk berkas cahaya laser diode dengan panjang gelombang 830 nm dikoreksi menggunakan sepasang prisma anamorpik. Penyangga kedua prisma dibuat agar pengaturan sudut keduanya dapat dilakukan dengan mudah. Pada penelitian ini variasi sudut kedua prisma adalah 10o dan 3o, 14,5o dan -1,5o, 18,5o dan -3o, 12o dan 1o, 6,5o dan 5o. Lebar berkas cahaya laser pada sumbu x dan y setelah melewati kedua prisma dengan lima variasi sudut diukur melalui pengukuran intensitas cahaya versus jarak dari penampang lintang berkas menggunakan sebuah fotodioda yang telah dikalibrasi. Dari grafik intensitas cahaya versus jarak, Full Wave Half Maximum (FWHM) dihitung sebagai lebar berkas. Gambar berkas cahaya sebelum dan setelah melalui kedua prisma pada kelima variasi sudut direkam dan dianalisa menggunakan kamera CCD untuk memperoleh bentuk berkas yang simetris. Dari kelima variasi sudut tersebut, variasi sudut yang ke empat dank e lima memberikan bentuk berkas cahaya laser yang hamper simetris atau bundar dengan lebar berkas di sumbu x dan y masing-masing (1,95 mm ; 1,75 mm) untuk variasi sudut yang keempat dan (1,45 mm ; 1,3 mm) untuk variasi sudut yang kelima.Item Pcngembangan Sistem Penjepit Cahaya (Optical Tweezers) Sebagai Alat untuk Pemerangkapan dan Pengaturan Partikel(2013-02-18) Minarni; Rahmad, Muhammad; SugiantoOptical Tweezers (OT) adalah sebuah sistem optik digunakan untuk pemerangkapan dan pengaturan partikel. Diperkenalkan pada tahun 1986, saat ini penelitian OT berkembang pesat dan mempunyai aplikasi pada berbagai bidang iimu. Partikel - partikel yang diperangkap bervariasi baik benipa atom, molekul, bakteri, dan DNA. Secara sederhana sebuah OT dibangun dengan sebuah berkas cahaya laser yang difokuskan ke sekumpulan partikel yang indek biasnya lebih besar dari indek bias medium sekelilingnya, laser yang digunakan mempunyai panjang gelombang disesuaikan dengan ukuran dan jenis partikel yang diperangkap. Pada penelitian ini, sebuah sistem Optical Tweezers dibangun mengunakan Laser Dioda 50 mW dengan Panjang Gelombang 830 nm. Partikel yang diperangkap adalah partikel Polystyrene berukuran 3 |j,m dan 10 (im. Lensa objektif lOOx, NA 1.25 merk Edmund Optics digunakan untuk memfokuska cahaya laser ke kaca preparat yang berisi partikel. Mikroskop optikal merk Leybold dimodifikasi dan dibuat menjadi digital mengunakan Kamera CMOS Thorlabs dengan Lensa 35 mm. Pencitraan partikel dan fokus laser pada kaca preparat sangat baik. Pada sistem ini, karena fluktuasi arus dan suhu ruangan yang tidak stabil menyebabkan daya laser sebelum menuju lensa objektif hanya sebesar 23 mW. Partikel 3 iim dapat terperangkap dengan baik sementara partikel 10 fxm membutuhkan daya laser yang lebih besar. Partikel-partikel yang tidak sepenuhnya mengalir atau terapung di dalam kaca preparat dan penampang berkas yang tidak sepenuhnya bulat menyebabkan Perangkap Optical tweezer yang tidak simetri sehingga gaya tarik pada partikel tidak begitu kuat.Item PENENTUAN MASSA MOLAR BEBERAPA JENIS LOGAM MENGGUNAKAN HUKUM DULONG-PETIT(2013-07-13) Simbolon, Henni; Minarni; Surbakti, AntoniusThe research about molar mass of some metals using calorimeter and Dulong-Petit’s law has been done. The metals used are Aluminium, Iron, Brass and Copper organized in a cubic form with mass of 2.79 g, 7.51 g, 8.38 g and 8.63 g respectively. The measurement of the heat capacity of calorimeter was conducted first as many as 7 times in order to include in the specific heat capacity. The specific heat capacity measurement of the metals was conducted first as many as 10 times for each metal. Molar mass of metals is then calculated using the specific heat capacity and law of Dulong-Petit. Research data shows that the temperature change of the water in calorimeter decreases overtime until it reaches the equilibrium while the temperature change of the metal and water mixture in the calorimeter increases overtime until it reaches the equilibrium temperature. Heat capacity of calorimeter measured is 67.35 J/0C. Specific heat capacities for metals Aluminium, Iron, Brass and Copper are 0.90 J/g0C, 0.47 J/g0C, 0.39 J/g0C and 0.38 J/g0C respectively. Calculation of the molar mass using the specific heat capacity and the law of Dulong-Petit are 27.96 g/mol, 53.53 g/mol, 64.54 g/mol and 66.40 g/mol respectively. Compared to the mass molar metal written on the Chemical Periodic Table, these discrepancies were found of 3.63% for Aluminium, 4.15% for Iron, and 1.58% for Copper.Item Pengembangan Sistem Penjepit Cahaya (Optical Tweezers) Sebagai Alat untuk Pemerangkapan dan Pengaturan Partikel(2012-10-29) Minarni; Rahmad, Muhammad; SugiantoOptical Tweezers (OT) adalah sebuah sistem optik digunakan untuk pemerangkapan dan pengaturan partikel. Diperkenalkan pada tahun 1986, saat ini penelitian OT berkembang pesat dan mempunyai aplikasi pada berbagai bidang ilmu. Partikel – partikel yang diperangkap bervariasi baik berupa atom, molekul, bakteri, dan DNA. Secara sederhana sebuah OT dibangun dengan sebuah berkas cahaya laser yang difokuskan ke sekumpulan partikel yang indek biasnya lebih besar dari indek bias medium sekelilingnya, laser yang digunakan mempunyai panjang gelombang disesuaikan dengan ukuran dan jenis partikel yang diperangkap. Pada penelitian ini, sebuah sistem Optical Tweezers dibangun mengunakan Laser Dioda 50 mW dengan Panjang Gelombang 830 nm. Partikel yang diperangkap adalah partikel Polystyrene berukuran 3 m dan 10 m. Lensa objektif 100x, NA 1.25 merk Edmund Optics digunakan untuk memfokuska cahaya laser ke kaca preparat yang berisi partikel. Mikroskop optikal merk Leybold dimodifikasi dan dibuat menjadi digital mengunakan Kamera CMOS Thorlabs dengan Lensa 35 mm. Pencitraan partikel dan fokus laser pada kaca preparat sangat baik. Pada sistem ini, karena fluktuasi arus dan suhu ruangan yang tidak stabil menyebabkan daya laser sebelum menuju lensa objektif hanya sebesar 23 mW. Partikel 3 m dapat terperangkap dengan baik sementara partikel 10 m membutuhkan daya laser yang lebih besar. Partikel-partikel yang tidak sepenuhnya mengalir atau terapung di dalam kaca preparat dan penampang berkas yang tidak sepenuhnya bulat menyebabkan Perangkap Optical tweezer yang tidak simetri sehingga gaya tarik pada partikel tidak begitu kuat.Item Pengembangan Sistem Penjepit Cahaya (Optical Tweezers) Sebagai Alat untuk Pemerangkapan dan Pengaturan Partikel(2013-04-01) Minarni; FitmawatiSelama dekade terakhir, jumlah penelitian pengembangan Penjepit Optical Tweezers (Penjepit Optik) yang portable dan ekonomis dan pengembangan aplikasi Optical Tweezers pada berbagai bidang ilmu meningkat sangat tajam. Optical Tweezers adalah alat yang mampu memerangkap dan mengatur partikel dari ukuran yang sangat kecil seperti DNA sampai ukuran yang besar seperti bakteria. Gaya cahaya atau radiation force diperoleh dari sebuah berkas cahaya laser yang difokuskan pada kumpulan partikel yang berada dalam sebuah kaca preparat. Sebuah Optical Tweezers sederhana terdiri dari sinar laser, pengatur posisi partikel dan alat pencitraan seperti kamera CCD beserta komputer. Panjang gelombang laser yang digunakan disesuaikan dengan ukuran dan jenis partikel yang diperangkap. Pada Penelitian Tahun ke III ini, sistem Optical Tweezers yang telah dibangun mengunakan Laser Dioda dan sebuah mikroskop Optik Merk Leybold di bandingkan performancenya dengan sebuah Optical Tweezer yang lebih kompak, portable mengunakan beberapa laser dioda dengan daya dan panjang gelombang yang berbeda yaitu dua laser dioda dengan Fiber Optic = 785 nm, P = 30 mW dan = 635 nm, P = 5 mW, dua laser dioda tanpa Fiber Optic dengan = 830 nm, P = 50 mW dan = 532 nm, P = 50 mW. Untuk Laser dioda-dioda tersebut, sebuah sistem yang terdiri dari tiga rangkaian pengatur arus untuk tiga laser dioda dibuat dan dianalisa, sebuah rangkaian penguat untuk Quadrant Photo Diode (QPD) dibuat, dimana Rangkaian QPD nya akan digunakan untuk mengkalibrasi Optical Tweezers. Dua set Optical Tweezers ini akan digunakan untuk menganalisa dua hal yaitu pengaruh panjang gelombang terhadap konstanta gaya dari Optical Tweezers dalam memerangkap partikel polystyrene dan pengaruh panjang gelombang terhadap fluoresence dari klorofil daun mangga.Item Pengembangan Sistem Penjepit cahaya (optical Tweezers) Sebagai Alat Untuk Pemerangkapan dan Pengaturan Partikel(2012-10-23) MinarniPenjepit cahaya, (optical Tweezers) telah menjadi sebuah alat yang mampu memerangkap dan mengatur partikel dari ukuran yang sangat kecil seperti DNA maupun ukuran yang besar seperti bakteria.Item Pengukuran Panjang Gelombang Cahaya Laser Dioda Menggunakan Kisi Difraksi Refleksi dan Transmisi(2013-07-13) Lestari, G.; Minarni; SaktiotoDiode laser is a laser with a semiconductor material. Nowdays, it is available at a relatively low price in different wavelength. The wavelength can be measured using the diffraction method with diffraction grating. In this research there are two types of diffraction grating i.e a reflection grating using a compact disc (CD) and a transmission grating from Phywee with 300 slits/mm. They were used for measuring the wavelength of four diode lasers with different wavelengths. The CD used are two empty CDs with the capacity of 720 MB and 700 MB, respectively. In order to be used as a reflection grating, the distance between pits in the CD must be known as a slit width. The distance was measured using a He-Ne laser light with a stable wavelength of 632,8 nm. The research results showed that the distance between pit width obtained are 1463,6 6,2 nm and 1454,4 4,1 nm for each CD. The measurement using the transmission grating has a lower standard deviation than the measurement using the reflection grating i.e 532,0 0,7 nm and 532,4 1,8 nm; 633,4 1,67 nm and 637,8 3,1 nm; 834,1 3,1nm and 835,3 5,1 nm, in contrast with an infrared laser (Near IR) with standard deviations lower for the reflection grating which are 786,7 1,7 nm and 784,4 2,5 nm. These values are comparable to the values listed on the packaging of each Laser which are Green Laser 532 nm, Red 638 nm, Infrared (Near IR) 785 nm and Infrared 830 nm.Item RANCANG BANGUN PENGATUR ARUS UNTUK TIGA PANJANG GELOMBANG LASER DIODA YANG BERBEDA(2014-03-26) Fitra, Ramma Hayu; Minarni; Setiadi, Rahmondia NandaA compact and low cost of Optical Tweezers usually uses diode lasers. Diode lasers are widely used because of their small size, broad wavelength variations and low cost. Howover, they have some disadvantages such as laser elliptical beam shape and vulnerable to electrical current fluctuations. In this research, a current regulator circuit system was designed and made which composed of a power supply circuit,three current regulators, and a current-limiting circuit. This system was used to regulate the electric currents injected into three laser diodes i.e type HL6312G with wavelegth 635 nm, type L780P01 with wavelegth 780 nm, and type WSLP-785-030m-4-PD with wavelegth 785 nm. From the results, variations of voltage and current versus time were obtained, electric current and power stability of the laser diodes versus time were observed for 60 minutes. Fluctuations obtained for all three circuits regulating electric current was about ± 0.001 mA. Variation of electric current for the laser diode to the three power laser diodes successfully obtained with current and power ranges were 12,2 mA to 76,6 mA and power from 0 mW to 4,55 mW for laser type HL6312G, current range 5,7 mA-40,0 mA and power from 0 mW to12,54 mW for laser type L780P010, current range 17,9 mA-129,9 mA and power from 0 mW to19,24 mW for laser type WSLP-785-030m-4-PD, respectivelyItem RANCANG BANGUN SISTEM DETEKSI FLUORESENSI KLOROFIL DAUN BAYAM BERBASIS FOTODIODA(2016-02-04) Afdlan, Muhammad Zaini; Minarni; ZulkarnainPlant interactions with the sun influence the amount of chlorophyll. The amount of chlorophyll influence the absorption of energy when excited by a laser. Design system of chlorophyll fluorescence detection based photodiode has been done. The chlorophyll of spinach leaf extract from spinach plants were given two different treatments used as a sample. Five variations of chlorophyll by spinach leaf concentration in an acetone used were 0,025gr/ml, 0.125 gr/ml, 0,25gr/ml, 0,375gr/ml, and 0,5gr/ml. Extract chlorophyll was excited by three laser diodes with different wavelengths are 405nm (violet), 532nm (green), and 780nm (red). Extract chlorophyll of fluorescence was detected using two photodiode which is connected to the amplifier circuit. Two photodiodes recording the light before recording the fluorescence of cuvette and extract chlorophyll. The output voltage of the amplifier circuit was measured using a multitester and the fluorescence spectra recorded by the CCD camera. The results showed that chlorophyll absorbs less energy when excited by green laser and red laser compared with as much as 50%, while the violet laser reaches 90%. Voltage output from amplifier circuit are influenced by variations of chlorophyll concentration.