Browsing by Author "Martina, A."

Now showing 1 - 5 of 5
  • No Thumbnail Available
    Item
    ISOLASI DAN SELEKSI JAMUR SELULOLITIK DARI HUTAN ARBORETUM UNIVERSITAS RIAU
    (2014-03-28) Hardianty, Denty Irma; Roza, R. M.; Martina, A.
    The Arboretum University of Riau constitutes of a secondary forest in Pekanbaru. Many plant genera exist in this Arboretum, therefore the litter produced has high content of cellulosa that may provide suitable environment for many cellulolitic fungus. The purpose of this study was to obtain the cellulolytic fungus isolates by screening the soil that collected from Arboretum University Riau, using Potato Dextrose Agar medium. The selection of cellulolytic fungus used Carboxyl Methyl Cellulose medium and was indicated by the appearance of clear zona. Cellulase activities were determinated using the ratio of clear zone and colony diameter. The result identified 53 cellulolytic fungus isolates. The L4H4 isolate had the highest ratio (3,51). There were three ratio criterions of clear zone to colony diameter in this study, i.e. high (24,53%), medium (35,85%) and low (39,62%). The characterization result showed that 15 fungus isolates belonged to the genera Aspergillus, Penicillium, Paecilomyces, Mucor and 2 isolates were unidentified
  • No Thumbnail Available
    Item
    POTENSI MIKROBA INDIGENUS ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU DALAM MENGHAMBAT PERTUMBUHAN Xanthomonas oryzae pv. oryzae
    (2014-03-28) Wizra, N.; Roza, R. M.; Martina, A.
    The plant disease caused by Xanthomonas oryzae pv. oryzae is still the major problem that can reduce agriculture productivity up to 20%. Currently the use of chemical bactericides can cause environment pollution and resistance to bacteria. Microorganisms can be used as biocontrol agent that is environmental friendly and potential to be developed. This study aimed to find the potential microbe from peat land at Rimbo Panjang village, Kampar Riau to produce antibacterial activity against Xanthomonas oryzae pv.oryzae. Antibacterial activity of microbe was tested using agar disk method and measured based on the ratio between the clear zone and colony diameter (ratio Z/K). The results showed 21 isolates of actinomycetes and 23 fungus isolates had antibacterial activity. The highest ratio activity for actinomycetes isolates was produced by Streptomyses RBIS6 (2.98) while the highest ratio for fungus isolates was produced by of Penicillium sp. L5J15 (2.00). Actinomycetes were the most potential microbes to produce antibacteria than the other microbes.
  • No Thumbnail Available
    Item
    POTENSI MIKROBA INDIGENUS ASAL TANAH GAMBUT DESA RIMBO PANJANG KABUPATEN KAMPAR RIAU DALAM MENGHAMBAT PERTUMBUHAN Ralstonia solanacearum
    (2014-03-28) Inggriani, Dwiana; Roza, Rodesia M.; Martina, A.
    Ralstonia solanacearum is one of soil pathogen bacteria that attack horticulture plant. Chemical pesticides are used commonly to control bacteria. Using chemical pesticides continously can give negative effect for living things and the evironment. Some soil microbes produce antibacterial compounds. This study aimed to test the potential of peat land microbes at Rimbo Panjang Kampar to inhibit Ralstonia solanacearum. This study used agar disk method to calculate the ratio activity between the inhibition zone and the colony diameter (Z/K) and then grouped into high, medium and low criteria. Twenty isolates of fungi that produced antibacteria against Ralstonia solanacearum consisted of genus Penicillium and Trichoderma. Isolate RPL2-29 (Penicillium) had the highest ratio (2.12) and isolate RPL2-38 (Penicillium) had the lowest ratio (1.14). Eleven isolates of actinomycetes that produced antibacteria consisted of Streptomyces, Micromonospora while 2 isolates has not been unidentified.Isolate RB3S51 (Streptomyces sp.) had the highest ratio (2.87) and RB3S57 (Streptomyces sp.) had the lowest ratio (1.29).The high criteria was dominated by actinomycetes isolates of the genus Streptomyces. Isolate RB3S51 (Streptomyces sp.) had the highest ratio (2.87) and the lowest ratio of fungal isolates RPL2-38 with ratio 1.14. Based on the characterization, the fungi were classified into the genus Penicillium
  • No Thumbnail Available
    Item
    UJI AKTIVITAS LIGNINOLITIK JAMUR PEKTINOLITIK TERMOTOLERAN INDIGENUS RIAU
    (2014-03-28) Febriani; Martina, A.; Roza, R. M.
    This study was aim to test the ligninolytic activity of 19 thermotolerant pectinolytic fungal isolates, which are indigenous from Riau. The selection of ligninolytic isolates was done using agar disk method. Isolates which showed ligninolytic activity were quantitatively tested using a completely randomized design with two factors: the temperature and isolates. The treatments in the first factor were the room temperature and 50 oC. The second factor were 5 isolates that had high ratio activity. The result showed that the ligninolytic activity at 50 oC was greater than at room temperature. Penicillium sp. PNE4 had the highest ratio activity (12.07) at 50oC. The temperature and isolates significantly affected in decreasing Poly R-478 concentration. Trichoderma sp. PNE13 isolate had the highest activity in degrading lignin than the other isolates at room temperature (46.52%) and 50°C (47.92%). Temperature affected the weight of biomass significantly. The biomass at room temperature was higher than 50 oC. The highest biomass at room temperature was produced by Aspergillus sp.2 (0.555 g/L), while the highest biomass at 50 oC was produced by Penicillium sp. PNE4 (0.359 g/L)
  • No Thumbnail Available
    Item
    UJI AKTIVITAS SELULASE JAMUR PEKTINOLITIK TERMOTOLERAN INDIGENUS RIAU
    (2014-03-28) Mira; Martina, A.; Roza, R. M.
    The indigenous isolates from Riau were known to have pectinolytic activity and thermotolerant, but their cellulase activity is still unknown. This study was aim to test the cellulase activity of 19 thermotolerant fungal isolates which are indigenous from Riau. The selection was done by using agar disk method. The determination of cellulase activity and biomass used a factorial complete randomized design (CRD) that consisted of two factors i.e. temperature and isolate. The result showed cellulase activity at 50°C was higher than at room temperature. Aspergillus fumigatus TT had the highest cellulolytic activity whereas Aspergilus sp.2 had the lowest cellulolytic activity. Temperature and isolates affected cellulase activity. Penicillium sp. PNE17 at 50°C had the highest cellulase activity and no significantly difference than Acremonium sp. PNE10. Temperature and isolates affected the biomass significantly but no interaction. Penicillium PNE17 had the highest biomass at room temperature (0,835 g/L) and at 50°C (0,330 g/L)