Browsing by Author "Ling, Ang Pay"

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    Molecular Cloning and Phylogenetic Analysis of Vitellogenin Gene in Hemibagrus nemurus
    (2016-08-28) Ling, Ang Pay; Harmin, S.A.; Salwany, M.Y. Ina; O. Roshani; Z. Zulperi; S.N. Baharum; Syahril, M.Z. Mohd
    Vitellogenin is an egg yolk precursor glycophospolipoprotein, which it can be used as a biomarker indicator in sexual maturation. In this study, total of the nucleotide size for this partial VTG sequence is 574bp. The partial VTG nucleotide homology of Hemibagrus nemurus is high; showing 88-91% identity between Clarias macrocephalus and Ariopsis felis. The VTG sequences analysis has shown the highest homologous portion of the VTG gene between local H. nemurus with Ariopsis felis and Bagre marinus. VTG from different species are conserved domains, and phylogenetically related. Studies as such will certainly provide new insights to better understand the biological role of VTG in river catfish.