Browsing by Author "Kurniasih"
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Item A NEW SPECIES OF CLINOSTOMUM (DIGENEA: CLINOSTOMIDAE) FROM CLIMBING PERCH (Trichogaster trichopterus) IN RIAU, INDONESIA(2013-05-30) Riauwaty, Morina; Kurniasih; Prastowo, Joko; WindartiThe aim of study is to identify the Clinostomum sp.obtained from visceral organs of Climbing perch (Trichogaster trichopterus) in Riau, Indonesia. In September 2010, Climbing perch (Trichogaster trichopterus) in Riau, Indonesia were collected, identified and examined for Clinostomum infection. Digenea, encysted metacercariae of Clinostomum were excysted with a needle, fixed in 10% formalin solution for morphological examination. Some samples were fixed with glutaraldehyde for SEM, and with ethanol absolute solution for molecular analysis. The result showed that the metacercariae of Clinostomum sp. from Riau had a body shaped like leaf. The surface of the body had not spinae. The anterior part was narrower than posterior. Posterior end was round. Body length had size of 347.2 (298.6 to 395.8) μm, body width of 127.2 (115.7 to 138.6) μm. Oral sucker small, terminal and had size of 16.2 (14,29 to 18,6) μm. Ventral sucker larger than oral sucker and had size of 63,6 μm. Clinostomum sp. has similarities to Clinostomum phalacrocorasis based on molecular study. This research was the first investigation of Clinostomum sp. in Climbing perch (Trichogaster trichopterus) as second intermediate host found in Riau, Indonesia.Item PREVALENSI CLINOSTOMUM (DIGENEA, CLINOSTOMIDAE) PADA IKAN SEPAT RAWA (Trichogaster trichopterus) DARI RIAU, INDONESIA(2013-05-30) Riauwaty, Morina; KurniasihThe aim of study was to know the prevalence of Clinostomum infections in Climbing perch (Trichogaster trichopterus) in Riau, Indonesia. Trichogaster trichopterus from Sail river, Riau were collected, and examined for Clinostomum infection. Encysted metacercariae of Clinostomum were excysted with a needle and fixed in 10% formalin. Speciemens were stained with Semichon’s Acetocarmine, dehydrated in alcohol series, cleared with xylene and mounted in entellan. The Clinostomum parasites were observed under a stereomicroscope and numbers recorded. Clinostomum identification was based on Chung et al 1995. The result showed that 18 of 105 observed climbing perch were infected by Clinostomum sp. The infected fish had total length between 5,6-11cm, while the weight range was 2,01-28,64 g. Based on the sexuality, the male fish was infected by the Clinostomum sp. (76,2%) more than the female (23,8%). Prevalence of Clinostomum sp. infection to the fish was 17,1%, and the infestation was between 1 to 29 worm per location. This research was the new record of Clinostomum sp. in Climbing perch (Trichogaster trichopterus) as second intermediate hostfound in Riau, Indonesia