Browsing by Author "Hartana, Alex"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    GENETIC DIVERSITY OF INDONESIAN MANGOES (MANGIFERA INDICA L.) USING RAPD MARKERS
    (2014-03-04) Fitmawati; Hartana, Alex; Purwoko, Bambang S
    Mango was introduced to the Caribbean in the XVIIIth century and became a popular garden tree. A regional programme for the inventory and conservation of fruit genetic resources undertaken in the French West Indies allowed the collection of 128 land races of mango in the Guadeloupe archipelago and in Martinique. Labelled accessions were selected according to local names, location and morphology. Microsatellite markers were developed for studying genetic diversity within this sample and within the germplasm bank maintained in Guadeloupe (Cirad) and for detecting duplicates. Nineteen microsatellite markers were selected and used to analyse a total of 307 accessions from India, South-East Asia, Florida, Africa and the Caribbean. Diversity was high within the sample with a total number of 140 alleles displayed. Results demonstrated the presence of duplicates in the germplasm bank and among the collected accessions, helping genetic resources management. Nevertheless, collecting efficiency was satisfactory with 73% of the material displaying distinct genetic profiles. Dissimilarities were calculated and a diversity representation was constructed using Neighbour Joining methodology. Accessions clustered in accordance with their geographical origin and their known history. The collected Caribbean accessions displayed a high variability, but shared some specific alleles and clustered together along with cultivars grown in Central (Mexico) and South America (Colombia) introduced from the South-East Asia, but also with cultivars from former French colonies in the Indian Ocean, indicating two introduction routes of mango to the French West Indies
  • No Thumbnail Available
    Item
    KARAKTER ROOT RE-GROWTH SEBAGAI PARAMETER TOLERANSI ALUMINIUM PADA TANAMAN PADI
    (2014-03-05) Roslim, Dewi Indriyani; Miftahudin; Suharsono, Utut; Aswidinnoor, Hajrial; Hartana, Alex
    Aluminum (Al) is one of the major limited factors in crop production on acid soils. Aluminum tolerant plants can be selected from plant breeding program by one of the physiological parameters representing Al tolerance character, such as root re-growth capability during recovery from the Al-stress. In this study we determined the concentration and time exposure of Al stress that was able to differentiate the response of three local upland rice varieties (Grogol, Hawarabunar and Krowal) and an Al-sensitive rice variety (IR64) to Al-stress, and evaluated the effectiveness of root re-growth (RRG) characters as an Al tolerance parameter in rice. The study consisted of three experiments, which were 1) nutrient culture experiment with different Al concentration treatments in growth chamber, 2) pot experiment in greenhouse using Jasinga yellow red podzolic acid soil containing 26,66 me/100 g Al and pH 4,6 as planting media, and 3) phenotyping of F2 population using RRG character. The results showed that Al treatment at 15 ppm for 72 h was able to distinctly differentiate between Al-tolerant (Grogol and Hawarabunar) and Al-sensitive varieties (Krowal and IR64). Planting of the rice varieties on acid soils showed similar result as that of the nutrient culture. Phenotyping of F2 population using RRG character indicated the existence of RRG value variation. These variations demonstrated that RRG character can be used as an Al tolerance parameter in rice and therefore can be effectively applied to screen rice F2 population that segregate to Al tolerance character.