Browsing by Author "Eryanti, Yum"

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    ANALISIS KROMATOGRAFI LAPIS TIPIS EKSTRAK ETANOL – SELULASE KULIT MANGGIS
    (wahyu sari yeni, 2019-01-14) Puja, Krisna; Nugroho, Titania T; Eryanti, Yum
    Extract of mangosteen fruid rind in ethanol - cellulase has high antioxidant activity. The identity of the high antioxidant activity is not yet known. The purpose of this study was to analysed the separation of this extract for further analysis. In this study, the analysis was carried out by thin layer chromatography technique with hexane : ethyl acetate (7 : 3) eluent. The results showed that this extract showed 4 number of spots at Rfs 0,07 ; 0,40 ; 0,60 and 0,73 detected by 254 nm UV light. Extract of mangosteen fruid rind in ethanol – cellulase was separated into several coumpounds based on its polarity
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    ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL TUMBUHAN KITOLOD (Isotoma longiflora (Wild.) Presl) TERHADAP Bacillus subtilis DAN Pseudomonas aeruginosa
    (2016-05-10) Paramita, Shinta; Eryanti, Yum; Teruna, Hilwan Yuda
    Isolation and antibacterial assay of Isotoma longiflora (Wild.) Presl plants have been done. The isolation of secondary metabolites of I. longiflora (Wild.) Presl was carried out by maceration method using n-hexane and methanol as solvent. A total of 500 g I. longiflora (Wild.) Presl plants yielded 23.31 g and 55.43 g of n-hexane and methanol extract, respectively. The methanol extract was fractionated using VLC and column chromatography. Antibacterial assay was conducted by the agar diffusion method against Bacillus subtilis and Pseudomonas aeruginosa. The methanol extract and fraction exhibited weak activity against two bacterial tested.
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    Isolasi Dan Uji Aktivitas Antuvflkrobial Ekstrak Daun Pacar Jawa (La Wsonia Inermis Linn)
    (2015-04-08) Yuharmen; Eryanti, Yum; Nurbalatif
    Perkembangan pengobatan tradisional Indonesia akhir-akliir ini mengalami kemajuan yang pesat. Hal ini didukung oleh keanekaragaman hayati Indonesia yang melimpah. Salah satu kekayaan flora tersebut adalah tumbuhan Pacar jawa {Lawsonia inermis, Liim) atau yang lebih dikenal dengan tumbuhan Inai. Daun tumbuhan Pacar jawa setelah diisolasi dengan pelamt heksan dan metanol, kemudian difraksinasi dengan kromatografi kolom. Ekstrak heksan, ekstrak metanol dan hasil fraksinasinya dilakukan uji aktivitas antimikrobial dengan menggunakan metoda difusi agar. Dari hasil anahsa pendahuluan, tumbuhan Pacar jawa mengandung senyawa terpenoid/steroid, flavonoid dan fenolat. Hasil kromatografi kolom fraksi heksan didapat senyawa LiH yang mempunyai titik didili 130-13rc. Spektrum UV senyawa LiH memberikan serapan maksimum pada = 210 run dan dengan penambalian basa tidak terjadi efek batokromik. Data spektrum IR menunjukkan terjadinya vibrasi ulur OH pada 3430 cm"^, ulur C-O pada 1381 cm"\ 1047 cm"' dan ulur C=C pada 1650 cm'^ Dari hasU uji pereaksi Liebermann-Burchard, senyawa LiH merupakan senyawa golongan steroid dan dari data UV dan IR disimpulkan bahwa senyawa LiH mempunyai gugus OH dan ikatan rangkap C=C yang tidak berkonjugasi. Ekstrak heksan dan hasil fraksinasinya tidak aktif terhadap spesies bakteri dan jamur yang diuji.
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    ISOLASI DAN UJI TOKSISITAS EKSTRAK ETIL ASETAT DAUN Nerium oleander
    (2016-05-10) Fitria, Nelda; Teruna, Hilwan Yuda; Eryanti, Yum
    The isolation of Nerium oleander was carried out by maceration method using n-hexane and ethyl acetate. The ethyl acetate extract of N. oleander was fractionated by Vacuum Liquid Chromatography (VLC). The fractions with vial number 6 to 9 (Fga) from VLC was separated by column chromatography. The fractions with vial number 6 to 23 (Fgb) from column chromatography was separated by column chromatography. The fractions with vial number 66 to 69 (Fgc) from the second column chromatography was characterized using Ultraviolet-Visible (UV-Vis) and Fourier Transform InfraRed (FT-IR) methods. The toxicity of extract and fraction have been determined by using Brine Shrimp Lethality Test (BSLT) methods. The toxicity result showed that the ethyl acetate extract and fractions of VLC (F4, F5, Fga, F10, F11) have LC50 < 1000 ppm. It showed that extracts and fractions were toxic. However, the toxicity result showed that the fractions of VLC (F1, F2, F3) and Fgc have LC50 > 1000 ppm.
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    Isolasi Metabolit Sekunder Buah Tumbuhan Tabernaemontana Sphaerocarpa (Apocynaceae)
    (2013-02-27) Eryanti, Yum; Jasril
    Telah dilakukan penelitian tentang isolasi metabolit seknnder buah tumbuhan Tabernaemontana sphaerocarpa (Apocynaceae). Padatan senyawa yang diperoleh diberi nama TSl yang mempunyai Rf = 0.57 (etil asetat: metanol + 1 tetes amonia = 7:3), Rf = 0.66 (B:A:W = 4:1:5). Hasil karakterisasi UV memberikan dua puncak serapan masing-masing pada panjang gelombang 245 ran (A = 1.784) dan 274.8 nm (A = 0.875) menunjukkan adanya sistem ikatan TI terkonjugasi dan senyawa TSl juga mengandung cincin aromatik. Spektrum IR menunjukkan adanya pita serapan pada billangan gelombang 3260 cm'' (N-CK3) atau (-OH), serapan 2953 cm' untuk ulur C-H aromatik, 1587 cm' menunjukkan adanya ikatan rangkap C=C. Spektrum ' H - NMR menunjiikkan adanya II dari cincin aromatis pada pergeseran kimia 5 sekitar 6.4-7.7 ppm, H dari C=C pada pergeseran kimia 6 = 4.29 ppm, H dari metil dan metilen pada pergeseran kimia 6 = 1.28-1.79 ppm dan H dari N-CH3 pada pergeseran kimia 8 2.01-1.79 ppm.
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    PERBANDINGAN METODE SINTESIS ONE-POT SENYAWA 3-(3-BROMOFENIL)-5-(2-METOKSIFENIL)-1-FENIL-4,5- DIHIDRO-PIRAZOL
    (2018-03-07) Izzaty, Dini; Eryanti, Yum
    Pyrazoline is a five-ring heterocyclic compound with three carbon atoms and two adjacent nitrogen atoms. The presence of electron-rich nitrogen atoms causes these compounds have various biological activities, such as anticancer, antitumor and antioxidant. The pyrazoline (PF-3Br-2OMe) was synthesized by a one-pot three-component reaction between 2-methoxybenzaldehyde, 3-bromoacetophenone and phenylhydrazine with strong base catalyst (NaOH). The synthesis of pyrazoline compound PF-3Br-2OMe was done by comparison of microwave irradiation method, reflux and solvent free. The structure of synthesized compound were confirmed by UV, FTIR, NMR and HRMS spectra. It was concluded that the good method for synthesis of pyrazoline PF-3Br-2OMe in the case of yield was the reflux method, with a yield of 40.51%. While the method was superior in terms of reaction time was microwave irradiation method with reaction time of 1 minute and yield of 34.83%.
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    Sintesis Dan Aktivitas Antimikrobial Beberapa Turunan Pirazolin
    (2015-07-02) Zamri, Adel; Yuharmen; Eryanti, Yum
    Scnyawa turunan pirazolin telah lama dikenal mempunyai aktivitas biologi yang beragam seperti obat penenang, antihipertensi, sitotoksik. Ahli depsresi dan anti kanker. Beberapa diantaranya juga dilaporkan mempunyai aktivitas sebagai analgesic, antipiretik, dan antimikroba. Sifat antimikroba yang diiringi dengan dengan sifat analgesik dan antipiretik adalah sangat jarang ditemukan dalam satu senyawa, karena itu senyawa pirazolin menarik untuk diteliti dan dikaji lebiii lanjut. Untuk maksud tersebut diperlukan pirazolin dalam jumlah yang cukup dan variasi struktur yang lengkap. Hal ini sulit diperoleh melalui isolasi dari dari bahan alam (tumbuhan dan hewan), karena itu sintesis merupakan salah satu cara yang efektif untuk membantu penyediaan senyawa tersebut dalam jumlah yang diinginkan. : Dalam jangka panjang penelitian ini bertujuan untuk membentuk perpustakaan molekul pirazolin. Target khusus yang ingin dicapai pada tahap ini adalah menghasilkan 15 molekul pirazolin beserta sifat fisiko-kimia dan aktivitas antimikrobanya. Pada kesempatan ini telah dilakukan sintesis turunan pirazolin dan sekaligus dilaporkan sifat fisiko-kimianya dan sifat biologinya sebagai antimikroba.
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    Sintesis dan Bioaktivitas Turunan Kurkumin dan Calkon
    (2013-04-17) Hendra, Rudi; Eryanti, Yum; Yuda Teruna, Hilwan; Yuharmen; Nurbalatif
    Telah dilakiikan sintesis dua turunan kurkumin yaitu (2E,6E)-2,6-bis(4- kloropenil)siklohenon dan (2E,6E)-2,6-bis(4-fluoropenil)sikloheksanon, dari suatu aldehid aromatik dan turunan keton dalam suasana basa melalui reaksi kondensasi aldol. Semua senyawa hasil sintesis sudah dikarakterisasi dengan spektroskopi UV, IR dan NMR.
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    SINTESIS DAN KARAKTERISASI SENYAWA (E)-3-(2-BROMOFENIL)-1-(NAFTALENIL)-2- PROPENON
    (2016-05-10) Safitri, Ariesta; Jasril; Eryanti, Yum
    A chalcone (E)-3-(2-Bromo fenil)-1-(Naftalenil)-2- Propenon were synthesized by Claisen–Schmidt condensation of 1-acetilnaphthalene with 2-bromobenzaldehyde using NaOH as catalyst. Its structures of compound were characterized based on the interpretation of spectroscopic data including UV, FTIR, NMR, and MS. The compound show a good yield (60,57%). Its melting point observed in the range of 102-103 0 C.
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    SINTESIS DAN KARAKTERISASI SENYAWA ANALOG PIRAZOLIN 5-(2-BROMOFENIL)-3-(NAFTALEN-2-IL)-1-FENIL-4,5-DIHIDRO-1H-PIRAZOL
    (2016-05-16) Lestari, Santy; Eryanti, Yum; Jasril
    Pyrazoline analog compound, 5-(2-bromophenyl)-3-(naphthalen-2-yl)-1-phenyl-4,5-dihydro-1H-pyrazole (PF CN2-2Br) has been synthesized through cyclization reaction between chalcone analog compounds and phenylhidrazine using acetic acid glacial as catalyst with microwave. A compound of chalcone analog, (E)-3-(2-bromophenyl)-1-(naphthalen-2-yl)prop-2-en-1-one (CN2-2Br) has been synthesized through Claisen-Schmidt reaction using irradiation microwave from 2-acetylnaphtalene and 2-bromobenzaldehyde using NaOH 1N as catalyst. PF CN2-2Br compounds structure was characterized using spectroscopy UV, IR and MS and it’s showed a good yield. PF CN2-2Br compound yields was 60,39%.
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    SINTESIS DAN MOLECULAR DOCKING SENYAWA (E)-7-(2-KLOROBENZILIDEN)- 3-(2-KLOROFENIL)-3,3a,4,5,6,7-HEKSAHIDRO-2H-PIRAZOLO[4,3-c]PIRIDIN-2- KARBOTIOAMIDA SEBAGAI ANTIDIABETES
    (Elfitra, 2022-06) Putri, Suci Mutiara; Eryanti, Yum
    Pyrazoline is a 5-heterocyclic compound with two nitrogen atoms known as an azole group which has various bioactivities, such as antidiabetic, antimicrobial, antioxidant and anticancer. For further development, pyrazoline (E)-7-(2-chlorobenzyliden)-3-(2-chlorophenyl)-3,3a,4,5,6,7- hexahydro-2H-pyrazolo[4,3-c]pyridine-2-carbotioamide was synthesized through Claisent- Schmidt condensation reaction between chloro-substituted curcumin with thiosemicarbazide and tested for antidiabetic bioactivity. The structure of the synthesized compound was confirmed by characterization using UV, FTIR, 1H-NMR and HRMS spectroscopy. Pyrazoline was tested for their antidiabetic activity through molecular docking and in vitro studies. Molecular docking studies was performed on the crystal structure of human lysosomal α-glucosidase (PDB ID: 5NN5) and compared it with acarbose as a positive control. The activity of the PZL-2Cl-KT compound was good activity in inhibiting the α-glucosidase enzyme, with an IC50 value of 83,93 μg/mL. However, it was confirmed by molecular docking studies was less good activity because that it only has one hydrogen bond interaction in common with acarbose, that is with the amino acid Asp518 and the bond free energy is -14.1723 kcal/mol compared to acarbose which is - 16.4969 kcal/mol.
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    SINTESIS DAN MOLECULAR DOCKING SENYAWA PIRAZOLIN (E)-7-(3- BROMOBENZILIDEN)-3(3-BROMOFENIL)-3,3a,4,5,6,7-HEKSAHIDRO-2HPIRAZOLO[ 4,3-c]PIRIDIN-2-KARBOTIOAMIDA SEBAGAI KANDIDAT ANTIDIABETES
    (Elfitra, 2021-12) Fenisia, Yediza; Eryanti, Yum
    Pyrazolin is a dihydropyrazole derivative which is an azole group compound with a 5-heterocyclic structure containing 2 nitrogen atoms. Pyrazoline is known to have several bioactivities, one of which is antidiabetic. Pyrazoline (E)-7-(3-bromobenzyliden)-3-(3-bromophenyl)-3,3a,4,5,6,7- hexahydro-2H-pyrazolo[4,3-c]pyridine-2- Carbotioamides are synthesized from bromosubstituted curcumin and thiosemicarbazide through the Claisent-Schmidt condensation reaction. The structure of the synthesized compound was confirmed by characterization using UV, FTIR, 1H-NMR and HRMS spectroscopy. Pyrazoline were tested for their antidiabetic activity through molecular docking and in vitro studies. Molecular docking studies were carried out on the crystal structure of human lysosomal α-glucosidase (PDB ID: 5NN5) and compared it with acarbose as a positive control. The activity of the pyrazoline PL-3Br-PN is classified as weak in inhibiting the α-glucosidase enzyme, which is 10.876% and confirmed by molecular docking studies that it only has one hydrogen bond interaction in common with acarbose, namely the amino acid His674 and a large bond free energy of -15,5423 kcal/mol compared to acarbose which is -21,3876 kcal/mol.
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    SINTESIS DAN STUDI MOLECULAR DOCKING SENYAWA 4-(3-(4-FLUOROFENIL)-5-(4-METOKSIFENIL)-4,5-DIHIDRO-1HPIRAZOL- 1-IL)BENZENSULFONAMIDA SEBAGAI INHIBITOR ENZIM TIROSINASE
    (Elfitra, 2022-01) Sitorus, Reforman; Eryanti, Yum
    Hyperpigmentation is a condition when melanin is produced in excess, causing dark patches to appear on the skin. Treatment of hyperpigmentation can be done through tyrosinase inhibition which will inhibit the formation of melanin. One of the compounds that has activity as an inhibitor of the tyrosinase enzyme is pyrazolin. The purpose of this study was to synthesize the compound 4-(3-(4-fluorophenyl)-5-(4-methoxyphenyl)-4,5-dihydro-1H-pyrazole-1-yl) benzensulfonamide, then to characterize the synthesized compound and test it. molecular docking. Pyrazoline compounds are synthesized by reacting 4-fluoroacetophenone and 4-methoxybenzaldehyde into chalcone through the clasen-schmidt reaction, then reacted with 4-hydrazinebenzensulfonamide which was also synthesized through diazotation and reduction reactions and continued the final reaction by reacting chalcone and hydrazine through a cyclization reaction to form pyrazoline. The structure of the synthesized compound was confirmed by UV spectroscopic analysis, FTIR, 1H-NMR, and HRMS. The yield obtained from the synthesis of pyrazoline was 78.11%. The synthesized pure compound was tested for activity as a tyrosinase inhibitor with a molecular docking study. Molecular docking tests were carried out on the crystal structure of tyrosinase (PDB:2Y9X) with natural ligands tropolone and kojic acid as positive controls. The docking results showed that the pyrazoline compound had a free bond energy (S score) = -10.5244 kcal/mol, while kojic acid had a free bond energy 2 (S score) = -8.7673 kcal/mol. The data that has been obtained indicate that the pyrazoline compound is predicted to be a potential inhibitor of the tyrosinase enzyme.
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    SINTESIS DAN UJI AKTIVITAS TOKSISITAS SENYAWA ANALOG KALKON TURUNAN 2’-METOKSIASETOFENON
    (2016-10-12) Junior, Ronaldo; Zamri, Adel; Eryanti, Yum
    Chalcone is one of secondary metabolites from flavonoids group that have been reported to posses many biological activities such as antimicrobial, antitumor, antioxidant, anti-inflammatory, antimalari and anticancer. Chalcone generally can be synthesized by Claisen-Schmidt condensation from aldehide and ketone aromatic using acid or base catalyst. Three hydroxy analogues chalcone i.e., (E)-3-(2-hidroksifenil)-1- (2’-metoksifenil)prop-2-en-1-on, (E)-3-(3-hidroksifenil)-1-(2’-metoksifenil)prop-2-en- 1-on and (E)-3-(4-hidroksifenil)-1-(2’-metoksifenil)prop-2-en-1-on have been synthesized under microwave irradiation by using pottasium hydroxide as catalyst. The structure of those compounds were characterized based on the interpretation of spectroscopic data included UV, FTIR, 1H-NMR and HRMS. Their toxicity activity were determined by using the Brine Shrimp Lethality Test method againts larvae of Artemia salina Leach and showed high activity with LC50 value was 66,59 μg/mL, 107,45 μg/mL and 449,44 μg/mL, respectivel. It indicated that chalcone analogues were potential as anticancer
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    SINTESIS DAN UJI ANTIBAKTERI SENYAWA (3E,5E)-3,5-BIS (4-HIDROKSIBENZILIDIN)PIPERIDIN-4-ON
    (2014-03-27) Sari, Tri Endah Tresna; Eryanti, Yum; Yuharmen; Zamri, Adel
    Curcumin is a colouring agent of Curcuma longa L. Beside the use of it as natural colourant, curcumin has biological activities such as antibacterial. In this research, curcumin analog compound of (3E,5E)-3,5-bis(4-hydroxybenzylidene)piperidin-4-one was synthesized with an acid catalyst (SOCl2) and the rendement obtained was 53.49 %. The purity of the compound has been tested using TLC, melting point test, and analytical HPLC. The identification of curcumin analogues was done using UV, IR, MS, 1 H NMR, and 13 C NMR. Antibacterial activity of the compound was identified by using disc diffusion method. The result showed that this analog of curcumin is inactive as an antibacterial agent.
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    Sintesis Dan Uji Bioaktivitas Beberapa turunan Kurkumin
    (2015-07-05) Eryanti, Yum; Yuharmen; Nurulita, Yuana
    Senyawa kurkumin baik sintetik maupun alami dikenal mempunyai aktivitas biologis yang bervariasi, seperti antioksidan, antibakteri, antiinflamasi, antimalaria dan antikanker. Aktivitas biologis dari senyawa kurkumin berhubungan dengan adanya keton a,p tak jenuh dan kekuatannya dipengaruhi oleh jenis dan pola substituen yang ada pada cincin aromatiknya. Pada laporan penelitian ini dilaporkan dua belas senyawa kurkumin telah berhasi! disintesis dan enam dari senyawa tersebut sudah dilakukan karakterisasinya. Senyawa analog kurkumin dibuat dengan cara merefluks turunan keton (asetofenon, siklopentanon, sikloheksanon), turunan aldehid aromatik (benzaldehid, sinamaldehid, 4- hidroksibenzaldehid,4-aminabenzaldehid) dan sodium hidroksida pelet dalam lumpang. Secara umum rendemen yang dihasilkan cukup tinggi (63-99%), ada tiga senyawa yang mempunyai hasil rendemen dibawah 75% yaitu senyawa yang mempunyai gugus pendorong elektron dalam hal ini adalah amin pada posisi para dari senyawa keton aromatik. Enam senyawa sudah dikarakterisasi dengan metoda spektroskopi UV, IR dan metoda NMR.
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    Sintesis dan Uji Bioaktivitas beberapa Turunan Kurkumin
    (2012-10-23) Eryanti, Yum
    Telah berhasil disintesis 12 senyawa turunan kurkumin dari empat senyawa aldehid(benzaldehid, sinamaldehid, para-hidroksi-benzaaldehid dan para-dimetilamina-benzaldehid)dengan tiga senyawa keton(aseton, siklopentanon dan sikloheksanon)melalui reaksi kondensasai claisen-schmidt dalam suasana basa.
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    Sintesis Dan Uji Bioaktivitas Beberapa Turunan Kurkumin
    (2015-07-05) Eryanti, Yum; Yuharmen; Nurulita, Yuana
    Senyawa kurkumin baik sintetik maupun alami dikenal mempunyai aktivitas biologis yang bervariasi, seperti antioksidah, antibakteri, antiinflamasi, antimalaria dan antikanker. Aktivitas biologis dari senyawa kurkumin berliubungan dengan adanya keton a,P tak jenuh dan kekuatannya dipengaruhi oleh jenis dan pola substituen yang ada pada cincin aromatiknya. Pada laporan penelitian ini dilaporkan dua belas senyawa kurkumin telah berhasil disintesis dan semua senyawa tersebut sudah dilakukan karakterisasinya. Senyawa analog kurkumin dibuat dengan cara merefluks turunan keton (asetofenon, siklopentanon, sikloheksanon), turunan aldehid aromatik (benzaldehid, sinamaldehid, 4- hidroksibenzaldehid, 4-aminabenzaldehid) dan sodium hidroksida pelet dalam lumpang. Secara umum rendemen yang dihasilkan cukup tinggi (63-99%), ada tiga senyawa yang mempunyai hasil rendemen dibawah 75%) yaitu senyawa yang mempunyai gugus pendorong elektron dalam hal ini adalah amin pada posisi para dari senyawa keton aromatik. Semua senyawa sudah dikarakterisasi dengan metoda spektroskopi UV, IR dan metoda NMR dan sepuluh senyawa sudah dilakukan uji aktivitas antioksidan, toksisitas dan antiinflamasi.
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    SINTESIS DAN UJI TOKSISITAS (2E,2'E)-3,3'-(1,4-FENILEN)BIS(1-(3- METOKSIFENIL)PROP-2-EN-1-ON
    (2020-05) Yulia, Sisi; Eryanti, Yum
    Chalcone is one of the secondary metabolites of the flavonoid group that has two aromatic rings connected by three carbon α, β unsaturated, the distribution of chalcone compounds in nature is very limited but has many important biological activities. In this research, symmetric chalcone (2E, 2'E) -3.3'- (1,4-phenylene) bis (1- (3-methoxyphenyl) prop-2-en-1-on chalcone was carried out and toxicity testing of these compounds. Chalcone compound was synthesized from the aldol condensation reaction between 3-methoxyacetophenone and terepthaldialdeid which was catalyzed by the NaOH base using stirring at 0-5 0C. The purity of the coumpounds was determined using by KLT test, melting point and HPLC analysis. Compounds structure was analyzed using UV spectroscopy, FTIR, 1H-NMR, and HRMS. The yield obtained from the synthesis is 31,25 %. Compounds toxicity test K1-3OMe determined by the BSLT method using shrimp larvae (Arthemia Salina Leach). LC50 value of compounds K1-3OMe is 216,27μg/mL. Concluded that the compound K1-3OMe are not toxic.
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    SINTESIS DAN UJI TOKSISITAS ANALOG DARI TURUNAN KALKON (E)-3-(4-HIDROKSIFENIL)-1- (2-METOKSIFENIL)-PROP-2-EN-1-ONE
    (wahyu sari yeni, 2019-08-15) Dani, Rahma; Eryanti, Yum
    Pyrazole is a important group of five ring heterocyclic alkaloid compounds containing two adjacent nitrogen atoms which have a diverse biological activities such as anti-inflammatory, analgesic, and antioxidant. Chalcone analogue of (E)-3-(4-hidroksifenil)-1-(2-metoksifenil)-prop-2en-1-one was synthesized via oxidative aromatization reaction with phenylhydrazine and catalyzed with glacial acetic acid. Chalcone was synthesized via microwave at 180 W and and pyrazoles were synthesized via reflux at 80oC. The purity of the compounds were determined by TLC, melting point and HPLC analysis. The structure of synthesized compounds were confirmed by UV, FTIR, NMR and HRMS spectroscopy studies. The toxicity of pyrazol was evaluated by Brine Shrimp Lethality Test (BSLT) method. Pyrazole PFC-4OH-2OMe considered toxicity as LC50= 165.95 μg/mL because the value of LC50 is less than 200 μg/mL