Browsing by Author "Devi, S."

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    Optimalisasi Komposisi Media dan Konsentrasi Sumber Karbon Produksi Enzim Selulase Bakteri Selulolitik Strain Lokal S-16 dan S-22
    (2013-03-07) Rahmi, F. L.; Dahliaty, A.; Devi, S.
    Isolates of S-16 and S-22 are some of the local strains producing cellulases that have been isolated from the Siak River water. Cellulase enzyme can be degrades of cellulose waste containing that enable for the processing of waste to economically priced products such as glucose. In this research, optimization of the composition production of cellulase enzymes from isolates of S-16 and S-22 with a variety of media production and concentration of carbon source production. The quantitative assay for enzyme activity was determined by Nelson Somogyi method with carboxymethyl cellulose (CMC) as a substrate. The highest cellulase enzyme activity of S-16 obtained in a media production (KH2PO4; MgSO4.7H2O; NaNO3; CaCl2; NaCl; CMC) of 88x10-3 U/mL, and the optimum concentration of source of carbon at 1% obtained for 42x10-3 U/mL, whereas of S-22 obtained in a media production (KH2PO4; MgSO4.7H2O; FeSO4.7H2O; NaNO3; KCl; CMC) of 14x10-3 U/mL, and the optimum carboxymethil Cellulosa concentration of sources of carbon at 1% obtained for 16x10-3 U/mL. Comparison of cellulase enzyme activity of isolate S-16 and S-22 by using Duncan test multiple range at level 5%
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    Optimalisasi Suhu dan Waktu Produksi Enzim Selulase dari Bakteri Selulolitik Strain Lokal S-16
    (2013-07-08) Simanjuntak, M. R.; Devi, S.; Dahliaty, A.
    Bacterial S-16 is one of the local strains of cellulolytic bacteria of biochemistry laboratory collection, FMIPA Riau University that is able to produce the cellulose enzyme. Cellulase enzyme is an enzyme that can hydrolyze the cellulose into reducing sugars. Production of enzyme from a microorganism is influenced by internal factors (genetic) and external such as temperature, pH, inducer compounds, carbon source and production time. In this study, the optimization of production of cellulase enzyme from S-16 was conducted in two phases : first optimization of the temperature variations of 25°C, 35°C, 50°C, 60°C and the second stage of the optimization of the production time variations 3, 6, 9, 12, 15, 18, 21, 24 o’clock at the optimum temperature obtained from the first stage. Produced cellulase enzyme activity was calculated based on reducing sugar formed from CMC substrate hydrolysis by cellulase enzymes with Nelson-Somogyi. Cellulase enzyme activity is equivalent to reduce sugar hydrolysis results. The results showed that the highest activity of cellulase enzyme bacterial S-16 was obtained at a temperature of 5°C which equal to 32x10-4 U/mL and the optimum production time at 21 hours by 60x10-4 U/mL. The highest protein content is at 15 hours of production time by 0,04413 mg/mL whereas cellulase activity and specific activity were not detected. Therefore, it is assumed that these bacterias produce another protein beside cellulase enzyme protein.
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    SELEKSI SEMBILAN BELAS BAKTERI ENDOFIT DARI UMBI TANAMAN DAHLIA(Dahlia variabilis) PENGHASIL ENZIM SELULASE
    (2014-03-27) Marlinda, S.; Devi, S.; Saryono
    Endophytic bacteria are bacteria that live in plant tissue and are unpathogenic to cell host. These bacteria can get into the plant tissue through injured plant tissue or bacteria can produce cellulase enzyme to degrade plant cell walls that contain cellulose.Cellulase is an enzyme that catalyzes the hydrolysis ofβ-1-4-glycosidic bond of cellulose. A total of nineteen isolates endophytic bacteria were inoculated onto medium containing 1% carboxymethyl cellulose (CMC). The six isolates have grown well which indicated that it has cellulase activity i.e. LBKURCC 45, LBKURCC 48, LBKURCC 53, LBKURCC 54, LBKURCC 59, and LBKURCC 60.