Browsing by Author "Dahliaty, A."

Now showing 1 - 5 of 5
  • No Thumbnail Available
    Item
    ANALISIS NUTRIEN KARBOHIDRAT, PROTEIN, LEMAK, SERAT KASAR DAN KALSIUM PADA KULIT BUAH SEMANGKA (Citrullus vulgaris, Schard) DI PEKANBARU
    (2013-08-01) Piliang, M.; Itnawita; Dahliaty, A.
    The watermelon pickles are wastes that have not been used optimally. The purpose of this study was to analyze the pickle nutrient content of some varieties of watermelon i.e seed less red, seed red, and yellow watermelon in order to increase the use of those pickles. The concentration of starch, protein, fat, crude fiber, and calcium of the pickles were determined by using Luff Schoorl method, lowry, soxhlet, acid-base hydrolysis and permanganometri titration, respectively. The results showed that starch content was ranged between 2,312% to 4,221%; protein content was ranged between 0,025% to 0,045%; fat content was ranged between 0,125% to 0,432%; crude fiber content was ranged between 1,678% to 2,403, and calcium content was ranged between 0,042% to 0,060%.
  • No Thumbnail Available
    Item
    KARAKTERISASI MEMBRAN SELULOSA BAKTERI Acetobacter xylinum HASIL FERMENTASI DAGING KULIT BUAH SEMANGKA
    (2014-03-27) Frenando, R.; Dahliaty, A.; Linggawati, A.
    Bacterial cellulose is be used as membrane filtration. This research was conducted to produce bacterial cellulose by using various concentrations of watermelon rind in the fermentation medium (30%, 40%, 50%, 60%, and 70%). The bacterial cellulose were produced then characterized for their morphology, functional groups, and selectivity analysis. Our result showed that the surface of the bacterial cellulose membrane and formed interwoven structure of microfibrils cellulose. The Cross-sectional observations of cellulose membrane showed many layers of bacterial cellulose with irregular pore and the layer had their own interbond. The FTIR spectra showed that the membrane has functional groups of C-H, O-H, C-O, C-O-C, and Pyranose which acted as specific functional groups of bacterial cellulose. These findings indicated that bacterial cellulose membrane of 70% watermelon rind concentration had the optimum selectivity value in 72.43% rejection.
  • No Thumbnail Available
    Item
    METODE ISOLASI FUNGI PENGHASIL KITINASE DARI SAMPEL TANAH
    (2014-03-27) Uliya, R.; Nugroho, T. T; Dahliaty, A.
    Chitinase producing fungi are potential biocontrol organisms for plant protection. This is due to the ability of chitinase to degrade the plant pathogenic fungi cell walls that contain chitin. Many soil samples contain biocontrol fungi. To obtain fungal isolates that produce chitinase from a soil sample, selective medium containing chitin as the sole carbon source should be used. This short paper describes a method to isolate chitinase producing fungi using a selective media. Using this simple method, three chitinase producing fungal strains could be isolated from a secondary forest located at the Giam Siak Kecil Bukit Batu biosphere reserve.
  • No Thumbnail Available
    Item
    Optimalisasi Komposisi Media dan Konsentrasi Sumber Karbon Produksi Enzim Selulase Bakteri Selulolitik Strain Lokal S-16 dan S-22
    (2013-03-07) Rahmi, F. L.; Dahliaty, A.; Devi, S.
    Isolates of S-16 and S-22 are some of the local strains producing cellulases that have been isolated from the Siak River water. Cellulase enzyme can be degrades of cellulose waste containing that enable for the processing of waste to economically priced products such as glucose. In this research, optimization of the composition production of cellulase enzymes from isolates of S-16 and S-22 with a variety of media production and concentration of carbon source production. The quantitative assay for enzyme activity was determined by Nelson Somogyi method with carboxymethyl cellulose (CMC) as a substrate. The highest cellulase enzyme activity of S-16 obtained in a media production (KH2PO4; MgSO4.7H2O; NaNO3; CaCl2; NaCl; CMC) of 88x10-3 U/mL, and the optimum concentration of source of carbon at 1% obtained for 42x10-3 U/mL, whereas of S-22 obtained in a media production (KH2PO4; MgSO4.7H2O; FeSO4.7H2O; NaNO3; KCl; CMC) of 14x10-3 U/mL, and the optimum carboxymethil Cellulosa concentration of sources of carbon at 1% obtained for 16x10-3 U/mL. Comparison of cellulase enzyme activity of isolate S-16 and S-22 by using Duncan test multiple range at level 5%
  • No Thumbnail Available
    Item
    Optimalisasi Suhu dan Waktu Produksi Enzim Selulase dari Bakteri Selulolitik Strain Lokal S-16
    (2013-07-08) Simanjuntak, M. R.; Devi, S.; Dahliaty, A.
    Bacterial S-16 is one of the local strains of cellulolytic bacteria of biochemistry laboratory collection, FMIPA Riau University that is able to produce the cellulose enzyme. Cellulase enzyme is an enzyme that can hydrolyze the cellulose into reducing sugars. Production of enzyme from a microorganism is influenced by internal factors (genetic) and external such as temperature, pH, inducer compounds, carbon source and production time. In this study, the optimization of production of cellulase enzyme from S-16 was conducted in two phases : first optimization of the temperature variations of 25°C, 35°C, 50°C, 60°C and the second stage of the optimization of the production time variations 3, 6, 9, 12, 15, 18, 21, 24 o’clock at the optimum temperature obtained from the first stage. Produced cellulase enzyme activity was calculated based on reducing sugar formed from CMC substrate hydrolysis by cellulase enzymes with Nelson-Somogyi. Cellulase enzyme activity is equivalent to reduce sugar hydrolysis results. The results showed that the highest activity of cellulase enzyme bacterial S-16 was obtained at a temperature of 5°C which equal to 32x10-4 U/mL and the optimum production time at 21 hours by 60x10-4 U/mL. The highest protein content is at 15 hours of production time by 0,04413 mg/mL whereas cellulase activity and specific activity were not detected. Therefore, it is assumed that these bacterias produce another protein beside cellulase enzyme protein.