Browsing by Author "Andrini, Fauzia"

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    METODE PCR DENGAN MENGGUNAKAN GEN COA UNTUK MENDETEKSI STRAIN STAPHYLOCOCCUS AUREUS PADA INFEKSI LUK A PASCA OPERASI PENDERITA DI RUANG RAWAT BEDAH
    (2013-03-05) Andrini, Fauzia
    Infeksi luka pascaoperasi merupakan salah satu infeksi yang paling sering terjadi pada infeksi nosokomial. Penyebab terpenting dari infeksi ini adalah S.aureus. Penentuan strain S.aureus hanya dapat dilakukan secara genotipik, yaitu dengan metode polymerase chain reaction (PCR) dengan menggunakan gen coa. Dilakukan pendeteksian strain S.aureus dengan metode konvensional pada isolat penderita dengan infeksi luka pascaoperasi dan didapatkan sebanyak 30 isolat yang positif S.aureus. Pada isolat-isolat tersebut dilakukan penentuan strain dengan cara mendeteksi gambaran hasil produk PCR dengan menggunakan gen coa, dan selanjutnya dilakukan prosentase tiap-tiap strain. Dari 269 penderita yang menjalani operasi 86 penderita (32%) mengalami infeksi luka pascaoperasi dimana 30 isolat (35 %) diantaranya disebabkan oleh S.aureus. Hasil PCR menunjukkan pita tunggal dan multipel dengan panjang antara 300 sampai 900 base pairs (bp). Infeksi luka pascaoperasi yang disebabkan oleh S.aureus pada penderita di ruang rawat bedah adalah disebabkan oleh 4 strain S.aureus yaitu strain 1 (1 pita, antara 800-900 bp) yaitu sebanyak 12 penderita (40 %), strain 2 (1 pita, mendekati 600 bp) sebanyak 11 penderita (36,7 %), strain 3 (A pita, mendekati 600 bp, antara 400-500 bp, mendekati 400 bp, mendekati 300 bp) sebanyak 5 penderita (16,7 %), dan strain 4 (2 pita, mendekati 300 bp, antara 400-500 bp) sebanyak 2 penderita (6,7 %).
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    Penentuan Genotipe dan Subtipe Virus Hepatitis B pada Penderita Hepatitis B Kronis, Sirosis dan Hepatoma di Pekanbara
    (2012-12-03) Zainal, Andi; Endriani, Rita; Andrini, Fauzia; M.Biomed, Arfianti
    Hepatitis B virus (HBV) infection can lead to diverse clinical spectrum of liver disease including fulminant hepatitis, chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC). HBV is currently classified into 8 genotypes (A to H) and 4 HbsAg-subtypes which are adw, ayw, adr, and ayr. Previous studies showed that HBV genotypes and subtypes showed a distinct geographic and ethnic distribution. Additionally, HBV genotypes may influence the clinical manifestation of chronic hepatitis B infection, development of HCC and response to antiviral treatment The aim of the present study was to investigate the differentiation of HBV genotypes and subtypes distribution among different clinical status of chronic hepatitis B in Pekanbaru. The design of present study was analytical cross sectional. HBV genotype was examined based on homology of S gene firom this study with that of GenBank Database. Gene S was generated by amplification using polymerase chain reaction (PCR) method, followed by DNA sequencing using dye-labelled terminator method. A total of 52 of chronic hepatitis B sera was examined in this study, vMch was obtained fiwm 10 of HBsAg-positive blood donors, 12 of chronic hepatitis B active patients, 10 of liver cirrhosis patients and 20 of HCC patients. The results showed that genotype C (59.6%) was the major genotype identified, followed by genotype B (40.4%). Based on clinical status, genotype C was predominantly found in blood donor (70%) dan liver cirrhosis patients (70%). While in chronic hepatitis B active and HCC, genotype B and C were comparable. Adr (59.6%) was the major HBsAg-subtype found in all subjects, followed by adw subtype (38.5%) dan ayw subtype (1.9%). Based on statistical analysis, there was no significant difference in the distribution of HBV genotypes and subtypes among blood donor, chronic hepatitis B active patients, liver ciniiosis patiraits and HCC. HOWCVCT, the distribution of HBV graiotype was significantly different between ciiriiotic-HCC patients and non cirrhotic-HCC patients. Genotype C (88.9%) was predominantly detected among cirriiotic-HCC patients, wliile genotype B (72.7%) was mostly idoitified among non cirrhotic-HCC patients (p=0.01).
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    PERBANDINGAN EFEKTIFITAS METODE CUCI TANGAN DENGAN SABUN ANTISEPTIK TERHADAP PENGGUNAAN LARUTAN MENGANDUNG ALKOHOL UNTUK ERADIKASI BAKTERI PADA TANGAN MAHASISWA KEPANITERAAN KLINIK SENIOR FAKULTAS KEDOKTERAN UNIVERSITAS RIAU
    (2013-03-01) Handayani; Andrini, Fauzia
    Perilaku mencuci tangan pada petugas kesehatan perlu ditingkatkan kaeran petugas kesehatan mudah untuk menyebarkan healthcare association infection (HCAI) dalam bekerja terhdap penanganan pasian terutam yany dirawat di ICU dan NICU. Perilaku mencuci tangan dapat mencegah peneybaran lebih dari 20 macam penyakit menular melalui kontak tangan.