Penentuan Aktivitas Enzim Selulase Dari Strain Lokal Aspergillus spp. Termotoleran

Abstract

Thermotolerant microorganism are microorganism that can live at temperatus of 20oC to 50oC temperature, therefore the enzymes produced by microorganism can be used in various industrial fields. One example is the enzyme cellulase produced by a local strain of Aspergillus spp. Cellulase enzymes to hydrolyze cellulase to break the bond β-1, 4 glycoside into a reducing sugar. The production of cellulase enzymes from the strain fungus Aspergillus sp1 TT, Aspergillus sp1, Aspergillus fumigatus KK, Aspergillus fumigatus KP and Aspergillus fumigatus TT uses a carbon source carboxymethyl cellulase (CMC) with two variations of temperature 45oC and 50oC. Cellulose enzyme activity assay was determined by Nelson-Somogyi method. The analysis showed the activity of cellulase enzyme from Aspergillus fumigatus KK at least 45oC higer temperatures (2,29x102±0,005) units/mL. At a temperature of 50oC cellulolase enzyme activity, where Aspergillus sp1 TT has the highest activity of cellulose enzymes by enzyme activity (1.38x10-3±0,0003) units/mL