ISOLASI DAN UJI ANTIOKSIDAN METABOLIT SEKUNDER EKSTRAK METANOL KITOLOD (Isotoma longiflora (Wild.) Presl)

Abstract

Isotoma longiflora (Wild.) Presl.is one species of medicinal plants found in tropical and subtropical regions. Isolation and qualitative antioxidant assay of I. longiflora have been done. The result of maceration yielded 23.31 g and 55.43 g of n-hexane and methanol extract, respectively. The fractionations of methanol extract was conducted by VLC, column chromatography, and column chromatography Sephadex LH-20. Qualitative antioxidant assay of methanol extract and fractions had been done using DPPH (1,1-diphenyl–2–picryl hydrazyl) method. The result of antioxidant assay showed that methanol extract, 3rd, 4th, 5th and 6th (F9-F11) fraction possessed antioxidant activity against DPPH radical. The purity of isolated compound was analyzed by HPLC. The chromatogram of HPLC showed that compound was not pure.