The Characteristic of Mitochondrial Cytochrome C Oxidase 1 Gene of Kryptopterus apogon (Bleeker, 1851)

Abstract

A study on sequence in the mitochondrial cytochrome c oxidase 1 gene of Kryptopterus apogon from Indragiri River of Riau Province In Indonesia has been conducted. This is a preliminary research on the mitochondrial cytochrome c oxydase 1 gene as molecular marker to obtain species diversity and and phylogenetic relationship on Kryptopterus from Indragiri River. Polymerase chain reactions (PCR) technique was applied to amplify cytochrome c oxidase 1 gene of 655 bp. The partial sequence of cytochrome c oxydase 1 gene was put on multiple alligment with other fishes in Siluriformes and Kryptopterus apogon from GenBank. Multiple alignment and analyzed using MEGA program version 3.0. Genetic distance based on nucleotide of cytochrome c oxidase 1 gene among Kryptopterus apogon (Indragiri river) and Kryptopterus apogon (GenBank) was 0.076. Their nucleotide substitutions consist of 42 nucleotide transitions and five nucleotide transversions. The phylogenetic relationship using Neighbor Joining method based on the sequence of nucleotide cytochrome c oxidase 1 gene among Kryptopterus apogon (Indragiri river) and Kryptopterus apogon (GenBank) form one phylogeny cluster with high bootstrap value.