Abstract:
Microalga has emerged as third generation in fuel based biomass which
converted into several products and its content can be also utilized as model compound.
Microalga comprises cellulose, hemicellulose and lignin. These cellulose and hemicellulose
can be converted into glucose, as model compound for fuel and chemical production. This
study focused on Tetraselmis chuii as feedstock and the hydrolysis pretreatment into
glucose conversion by employing diluted sulfuric solution in varied temperature and
concentrations. Tetraselmis chuii was used as microalga feedstock since they are distributed
in Indonesia’s ocean. In order to obtain fuel or chemical product, microalga need to be
pretreated first. This process was conducted with focused on breakdown the linkage
between cellulose, hemicellulose or lignin. Glucose was generated from this hydrolysis
process. Thus, it would apply as feedstock for bioethanol or furan production which utilized
as intermediate compound/building block compound. This study aimed into the significant
parameters for Tetraselmiss chuii hydrolysis and their biochemical composition.
Experimental result was assayed in quantitative method by using High Performance Liquid
Chromatography (HPLC). It was found out that Tetraselmiss chuii contain alpha cellulose
and hemicelluloses without lignin present. Moreover, temperature has significant
influenced for hydrolysis process than sulfuric acid concentration. The highest glucose
temperature was 61 g/L which obtained at 100oC by using 1.25%(v/v) diluted sulfuric acid
concentration.
