EFEK EKSTRAK ETANOL DAUN PELAWAN (Tristaniopsis obovata R.Br) TERHADAP STRUKTUR LIMPA TIKUS PUTIH (Rattus norvegicus) YANG MENGALAMI UROLITHIASIS

Abstract

This study is aimed to ascertain the effect of the ethanol extract of Pelawan plant (Tristanopsis obovata R.Br) toward Rattus norvegicus spleen tissue structure that had suffered urolithiasis. The experiments was design as Post Test only Control Group Design. Twelve males of R.norvegicus were divided into 4 groups, the first group was treated with Inducer (solution mixture of ethylene glycol 0.75% and ammonium chloride 2%), the second and the third group was treated with Inducer solution and ethanol extract of Pelawan leaves with a dose of 100 mg/kg BW and 150 mg/kg BW. The fourth group was considered as control and was not treated with inducer and ethanol extract of pelawan. All treatment were conducted 14 days. Histological slides were made using paraffin method and were stained using HE. The result was analyzed with one way ANOVA, followed by Tukey test if there is significant difference. Observation on spleen tissue indicated the damages on group treated with inducer, such as the hemorrhagic, the arteries dilatation, cell swelling, cytoplasm vacuolization, cell degeneration, necrosis pycnosis, and necrosis karyolysis. The recovery of spleen tissues were showed in group treated with Inducer + extract 100 mg/kg BW and Inducer + extract 150 mg/kg BW. They were artery normalization, cells degeneration, cytoplasm vacuolization, hemorrhagic area reduction, and hemosiderin formation. The result indicated that ethanol extract of Pelawan leaves have a positive effect on recovering the damages of spleen tissue caused by ethylene glicol