ISOLASI DNA DAN AMPLIFIKASI GEN COX3 PADA IKAN SELAIS KAPOREH (Ompok eugeneiatus Vaillant 1893) DARI SUNGAI KAMPAR, PROVINSI RIAU

Abstract

Selais Kaporeh (Ompok eugeneiatus) is an endemic fish in floodplain river. The genetic information of Ompok eugeneiatus is still limited. This research aims to isolate and amplify Cytochrome c Oxydase sub unit 3 (COX3) gene from Ompok eugeneiatus originated from Kampar River. This study was conducted from November 2014 to April 2015. Cytochrome c Oxydase sub unit 3 gene was amplified using COX3-F1 and COX3-R1 primers which were design from COX3 gene of Ictalurus punctatus (GenBank 2004). PCR condition based on Elvyra and Duryadi (2007) with modification. The result indicated that COX3-F1 and COX3-R1 primers can be used to amplify COX3 gene of Ompok eugeneiatus with size of 642 bp.