Abstract:
Department of Chemistry, Faculty of Mathematics and Natural Science, University of Riau,
Pekanbaru, Riau, Indonesia, 2Department of Agrotechnology, Faculty of Agricultural,
University of Riau, Pekanbaru, Riau, Indonesia, 3Department of Environment Microbiology,
Faculty of Technical, Bandung Institute of Technology, Bandung, West Java, Indonesia
Maria Lorenita, Yuli Haryani, Fifi Puspita, Didit Trihartomo and Saryono Sikumbang (2013)
Screening of endophytic fungi from tubers of Dahlia variabilis. Journal of Agricultural
Technology 9(3):585-590.
Researches on plant-derived endophytic fungi have grown in recent decades. Dahlia variabilis
is one of natural products which potential as a source of antimicrobial agents. The purpose of
this research was to isolate and characterize endophytic fungi from the tubers of Dahlia
variabilis because derivation of bioactive compounds directly from the plants needs too many
biomasses. Direct seeds method was used to isolate endophytic fungi from the samples.
Samples were surface-sterilized by immersing in 70% ethanol, soaked in 15% peroxide acid,
rinsed in sterile distilled water, and then put on the surface of Sabouraud-4% Dextrose Agar
(SDA). Four isolates of endophytic fungi were isolated and characterized morphologically i.e.,
Monilia sp., Aureobasidium sp., Moniliella sp., and Sporothrix sp.
