UJI AKTIVITAS LIGNINOLITIK JAMUR PEKTINOLITIK TERMOTOLERAN INDIGENUS RIAU

Abstract

This study was aim to test the ligninolytic activity of 19 thermotolerant pectinolytic fungal isolates, which are indigenous from Riau. The selection of ligninolytic isolates was done using agar disk method. Isolates which showed ligninolytic activity were quantitatively tested using a completely randomized design with two factors: the temperature and isolates. The treatments in the first factor were the room temperature and 50 oC. The second factor were 5 isolates that had high ratio activity. The result showed that the ligninolytic activity at 50 oC was greater than at room temperature. Penicillium sp. PNE4 had the highest ratio activity (12.07) at 50oC. The temperature and isolates significantly affected in decreasing Poly R-478 concentration. Trichoderma sp. PNE13 isolate had the highest activity in degrading lignin than the other isolates at room temperature (46.52%) and 50°C (47.92%). Temperature affected the weight of biomass significantly. The biomass at room temperature was higher than 50 oC. The highest biomass at room temperature was produced by Aspergillus sp.2 (0.555 g/L), while the highest biomass at 50 oC was produced by Penicillium sp. PNE4 (0.359 g/L)