ANALISIS TOTAL FENOLIK, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN PADA DAUN SERTA BATANG TANAMAN Coleus amboinicus Lour (BANGUN-BANGUN)

Abstract

The objective of this study was to analyze the total phenolics, flavonoids, and antioxidant activity of leaves and stems of C. amboinicus. Two different watering treatments (EM5 and FPE) were applied to C. amboinicus during cultivation. The results showed that leaves of C. amboinicus treated with FPE had the highest total phenolic and flavonoid content than other samples. The total phenolic content was 2.09 mg Gallic Acid Equivalent (GAE)/g of dry weight (DW) and the total flavonoid content was 6.218 mg Catechin Equivalent (CTE)/g of DW respectively. The leaves treated with FPE also had the highest antioxidant activity by FRAP test (0.058 mmol Fe+2/g DW). The highest antioxidant activity by DPPH test was found in the leaves treated with EM5 (1.42 mg Ascorbic Acid Equivalent (AAE)/g DW). From this study it can be concluded that leaves of C. amboinicus was the best source of antioxidant. FPE watering treatment gave a better influence on total phenolic, flavonoid, and antioxidant activity of C. amboinicus than EM5