Abstract:
The S-16 and S-22 cellulolytic bacterias isolated from Siak River water were used in this study to produce cellulase using sugarcane bagasse. Sugarcane bagasse contains ±
50% cellulose that can be used as a substrate in the production of cellulase. Cellulase is an enzyme that catalyses the hidrolisis of β-1-4-glycosidic bond of cellulose. The objectives of this study were to determine the optimum concentration of sugarcane
bagasse (0.25%, 0.5%, 1.0% and 1.5%) The results indicated that 1% bagasse substrate with 24 hours of production time gave the optimum enzyme activity. The enzyme
activity of S-16 and S-22 were 0,000233±0,00091 U/mL and 0,01259±0,00042 U/mL
