METODE ISOLASI FUNGI PENGHASIL KITINASE DARI SAMPEL TANAH

Abstract

Chitinase producing fungi are potential biocontrol organisms for plant protection. This is due to the ability of chitinase to degrade the plant pathogenic fungi cell walls that contain chitin. Many soil samples contain biocontrol fungi. To obtain fungal isolates that produce chitinase from a soil sample, selective medium containing chitin as the sole carbon source should be used. This short paper describes a method to isolate chitinase producing fungi using a selective media. Using this simple method, three chitinase producing fungal strains could be isolated from a secondary forest located at the Giam Siak Kecil Bukit Batu biosphere reserve.