Abstract:
Isolation, characterization, and in vitro testing against murine leukemia P-388 cell of flavonoid compounds from Bauhinia hullettii Prain stem bark have been
performed. Isolation was carried out by extraction and fractionation. Extraction used
meceration method at room temperature to obtain n-Heksana extract, dicloromethane
extract, and etil acetate extract. Based on phytochemical testing, etil acetate extract
contain flavonoid compounds. Extraxct etil acetate was fractionated by vacum
column chromatography, gravitation column chromatography, and flash column
chromatography. The fractionation resulted yellow crystal that having melting point
of 188
o
C - 189
o
C. The in vitro testing against murine leukimia P-388 cell of isolated
flavonoid compounds show “medium” activity with IC
50 = 22,5 μg/ml.
