IDENTIFIKASI DAN UJI TOKSISITAS EKSTRAK N-HEKSAN DARI KULIT BIJI TANAMAN SIRSAK (Annona muricata L)

Abstract

Annona muricata is a plant which is used as traditional medicine in Indonesia. Isolation from seed coat of Annona muricata has been done using macerating method with n-hexane and then it was fractionated by column chromatography and recrystalization. Analysis of fractionation result has been done using FT-IR spectrophotometry, UV-Vis and Gas chromatography-mass spectroscopy (GC-MS). BSLT method was used as an toxicity test at concentrate extract, n-hexane fraction and the result of recrystalization. Four dominant compounds at n-hexane extract are obtained from GC-MS. They are methyl palmitate, ethyl palmitate, methyl oleate and ethyl oleate. The toxicity test showed LC50 2,42 ppm, LC50 4,79 ppm and LC50 2,42 ppm for crude extract, hexane fraction and recrystalization compound respectively.