PENGARUH KOMBINASI GLIBENKLAMID DAN MINYAK BUAH MERAH (Pandanus conoideus Lam) TERHADAP TINGKAT KERUSAKAN TUBULUS RENALIS PADA TIKUS JANTAN GALUR WISTAR DIABETES

Abstract

ABSTRACT Diabetes mellitus refers to metabolic group disease which is characterized by chronic hyperglicemia resulting from impaired insulin secretion, insulin action or both. Diabetes mellitus complications affect many organs like kidney. Kidney abnormalities are due to hyperglicemia period and oxidative stress. Pandanus conoideus Lam oil contains β-carotene and α-tocopherol that can reduce oxidative stress. The aim of this study is to observe the effect of glibenclamide and Pandanus conoideus Lam oil combination on rat’s renal tubule. Diabetes was induced by streptozotocin (60 mg/kg;intraperitoneal). Rats were randomly divided into normal group, diabetic group, diabetic group administered glibenclamide and diabetic group administered combination of glibenclamide and Pandanus conoideus Lam oil. The animals were treated with glibenclamide and Pandanus conoideus Lam oil once daily for 14 days. The diabetic group showed hypertrophy of proximal cells tubule, Ebstein Armanni lession and proximal tubular dilatation. These features were reduced on diabetic group administered combination of glibenclamide and Pandanus conoideus Lam oil. This study concludes that combination of glibenclamide and Pandanus conoideus Lam oil administration can prevent the progression of renal tubular injury on diabetic rats.