ANALISIS NUKLEOTIDA GENA PENYANDI PROTEIN E6 HUMAN PAPILLOMA VIRUS TIPE 16 DARI PENDERITA KARSINOMA SERVIKS

Abstract

Background : Human Papilloma virus (HPV) infection has been known as the major cause of cervical cancer which represent the leading causes of morbidity among women woridvydde, E6 and E7 ORFs contain the tt^sforming abihlty of HPV and was the first indication of an important role for these genes in HPV associated tumorigenesis. Some of ihe changes in E6 may alter biological properties and protein function. The aim of this study is to analyze the sequence of the gene encoding E6 protein of HPV 16 fit)m Cervical Carcinoma patients at Dr. Sardjito Hospital Yogyakarta period October 2007-February 2008. Methods :Thirty one cervical cancer patients were studied. The viral DNA were isolated fi-om the cervical tissue using standard method. The viral type were determined by PGR using specific primers for HPV 16. The gene encoding E6 protein of HPV 16 were amplified using specific primers and sequenced using automated DNA sequencer machine. Results : after HPV typing, the entire HPV 16-E6 coding region was amplified and sequenced in all samples. We detected total 13 variants of nucleotide fi-om all sequence samples that caused a base substitution ih the E6 coding region, resulting in amino acid changes namely T109C, C116A, G124A, G132T, C143G, A144G, A276G, T286A, A289G, C335T, A403G, G489C, G491T. Compared to another sequence fi-om whole world, we detected the same nucleotide changes especially on Afiica type 1, Afiica type 2 and Australian strain. Conclusion: The entire HPV 16-E6 coding region had been amplified and sequenced successftjlly. There were nucleotide changes that caused polymorphisms of amino acid sequence.