Abstract:
Nosocomial Infection or Health Care Associated Infection (HCAI) is one of the
leading causes of mortality and morbidity in hospitals and the other health care
facilities. Washing hands is the way to reduces incidence of HCAI. World Health
Organization (WHO) recommends the use of alcohol-based handrub because its
antibacterial activity had been tested in clinical and WHO has identified formulations
for the local preparation. This is an experimental study aimed to determine the
antibacterial activity of World Health Organization (WHO)-recommended handrub
formulation and isopropyl alcohol 60% to 20 subjects. Each subject wash their hand
using WHO-recommended handrub formulation and isopropyl alcohol 60% and then
do the test of antibacterial avtivity and count the bacterial colonies by Total Plate
Count method. The results of this study showed that significantly log 10 of bacterial
colonies in pre and post-handrubbing using WHO-recommended handrub
formulation and isopropyl alcohol 60% and not-significantly difference of log 10
decrease using WHO-recommended handrub formulation and isopropyl alcohol 60%.
