PENGARUH ION LOGAM Cu2+ DAN Mg2+ PADA AKTIVITAS LAKASE FRAKSI FLOW THROUGH KROMATOGRAFI GEL FILTRASI

Abstract

Laccase Trichoderma asperellum LBKURCC1 has been partially purified fractionation using ammonium sulfate at 0-80% saturation then gel filtration chromatography. The results of the gel filtration have different activities and purity levels The fraction has lower activity and lower purity is called laccase flow through gel filtration chromatography. The activity of the laccase enzyme was tested using substrates 2,2'-azinobis-3- ethylbenzothiazoline- 6-acid sulfonate (ABTS) measured at a wavelength of 405 nm using a microplate reader. The activity of laccase flow through is 144,02±2,71 U/L. The laccase activity was increased significantly (p≤ 0.05) to (362 ± 48)% and (445 ± 59)% compared to controls, when the enzyme was incubated for 10 minutes with 0.5 mM and 5 mM Cu2+ respectively. A significant increase (p≤ 0.05) in laccase activity was also observed when the enzyme was incubated for 10 minutes in 0.5 mM and 5 mM Mg2+, The laccase activity was increased significantly (p≤ 0.05) to (343 ± 59)% and (219 ± 50)% compared to controls.