ISOLASI DAN UJI TOKSISITAS EKSTRAK ETIL ASETAT DARI BENALU JENGKOL Scurrula ferruginea

Abstract

Scurrula ferruginea belongs to the Loranthaceae family which lives on its host, the jengkol tree. Ethyl acetate is a solvent with low toxicity that is semipolar so it is expected to attract compounds that are both polar and non-polar. This research aims to isolate secondary metabolites and test the toxicity of the jengkol parasite plant from ethyl acetate extract using the brine shrimp lethality test (BSLT) method. The samples were extracted using the maceration method using methanol solvent and partitioned using n-hexane and ethyl acetate solvents. The ethyl acetate fraction was separated using vacuum liquid chromatography (VLC) which produced 7 fractions. Fractions 2 and 3 were separated using gravity column chromatography which produced 6 fractions. In fraction 5, the ethyl acetate extract produced a pure compound in the form of a white powder with a melting point of 279-281°C and codenamed SFEA-F5. The FTIR spectrum was obtained at wavenumber of 3356 cm-1 (OH), 2933 cm-1 ( aliphatic C-H), 1455 cm-1 (CH2), 1021-1166 cm-1 (C-O). Based on the results of the characterization of the SFEA-F5 compound, it is a class of terpenoid compounds. The results of the toxicity test with BSLT showed that the ethyl acetate extract was toxic with an LC50 value of 115,63 ppm.