ISOLASI DAN UJI TOKSISITAS SENYAWA METABOLIT SEKUNDER DARI FRAKSI ETIL ASETAT KULIT BATANG TUMBUHAN PULAI Alstonia scholaris

Abstract

Alstonia scholaris as known in Indonesia as pulai . A. scholaris is one of the plants that is widely used in traditional medicine in India and Southeast Asia including Indonesia. This study aimed to isolate and characterize pure compounds obtained from the ethyl acetate fraction of A. scholaris bark and test its toxicity using the BSLT (brine shrimp lethality test) method. Isolation is carried out by extraction methods in the form of maceration and partitioning. The separation of isolated compounds is carried out by flash column chromatography which produces crystals and is coded ASc_E_34. The crystal has a melting point of 128-130°C. UV-Vis spectrum of ASc_E_34(λ) compound 285 nm (A) 0.116. The IR spectrum has an absorption band at wavenumber 3587; 3566 cm-1 (N-H), 2941; 2849 cm-1 (aliphatic C-H), 1683-1512 cm-1 (C=C) and 1272;1143;1028 cm-1 (C-O alcohol), 847 cm-1 (C-H). Based on the results of the analysis, the resulting crystals are alkaloid compounds. The results of the toxicity test conducted stated that the sample was not toxic by showing LC50 > 1000 ppm values.