PENGARUH PENAMBAHAN ION LOGAM DAN PELARUT ORGANIK TERHADAP AKTIVITAS ENZIM LAKASE HASIL FRAKSINASI 20-80% AMMONIUM SULFAT

Abstract

Laccase is an enzyme that requires additional chemical components (non-protein) such as metal ions to increase its activity. This study aims to determine the effect of adding Cu2+, Mg2+ and Zn2+ metal ions and organic solvents acetonitrile, acetone, ethanol and methanol on the activity of the laccase enzyme produced from the fungus Trichoderma asperellum LBKURCC1 using ABTS as a substrate. The concentration of metal ions and organic solvents added were respectively (1, 5 and 10 mM) and (5, 20 and 40 %). Laccase enzyme activity test was measured using a UV-Vis spectrophotometer with a wavelength of 420 nm and incubated for 5 minutes. The highest laccase activity was obtained by adding Cu2+ metal ions at a concentration of 10 mM with a value of 544.00 ± 12.56 U/L, which increased laccase activity by 63,43% which acts as a cofactor. Meanwhile, the addition of organic solvents decreased its activity by 80,17% with a value of 66.00 ± 4.09 U/L in acetonitrile solvent with a concentration of 40% which caused the enzymes to denature.