Abstract:
Polyhydroxyalkanoate (PHA) as one of the polymers for making bioplastics had
potential to subtitute synthetic plastics that are environmentally unfriendly. By
optimization of PHA production using cheap and renewable substrates, production
costs can be reduced. In this study, modified palm oil mill effluet (POME) and
coconut water enriched media will be used to optimize the production of PHA by
soil bacteria from Riau. Cultures of bacteria were purified and selected using Sudan
Black B (SBB) techniques, and the selected isolates were used to optimize the
production of PHA with three different enriched media, 50% POME, 50% coconut
water, and 25% coconut water. Based on the results of bacterial purification and
selection, 6 of 19 isolates were highly absorbent. HTI3_3, HS4_1, KS4_1, and
TPAS1_5 isolates were selected to optimize production. The results showed the
highest dry weight and PHA accumulation were 1,49 g/L and 82,27%, respectively,
produced by isolate KS4_1 using 25% coconut water enriched media.
