Abstract:
Red cambodia (Plumeria rubra) is a plant of the Apocynaceae family which is rich in
secondary metabolites and has potential as a source of medicines. This study aims to
isolate secondary metabolites contained in red frangipani flowers extracted using ethyl
acetate as a solvent and to test antioxidant activity using the DPPH method. The purpose
of this study was to isolate secondary metabolites and test the antioxidant activity of red
frangipani flowers from ethyl acetate extract using the DPPH method. Phytochemical test
results showed the content of secondary metabolites of terpenoids, saponins, flavonoids
and phenolic groups. Isolation was carried out by maceration method using methanol as
solvent and partitioned using n-hexane and ethyl acetate as solvent. The ethyl acetate
extract was separated using Vacuum Liquid Chromatography (VLC) to produce 7
fractions. In fraction 4 the ethyl acetate extract produced a pure compound in the form of
a white powder with a melting point of 264-266°C and coded PrEF4. The FTIR
spectrophotometer indicates the presence of absorption in the wave number (cm-1) i.e.
3310 (OH), 2849-2959 (C-H), 1588 (C=C), 1463 (CH2), 1379 (CH3), and 1055 (C-O).
The results of the antioxidant test using the DPPH method showed that the ethyl acetate
extract had an IC50 191 μg/mL (AAI 0,42) value which was classified as weak and the
PrEF4 compound had an IC50 > 1000 μg/mL (AAI < 0,5) value classified as very weak
or inactive as an antioxidant.
