UJI AKTIVITAS BAKTERI LOKAL PENGHASIL LIPASE DENGAN MENGGUNAKAN INDUSER MINYAK JAGUNG

Abstract

Lipase is an enzyme that is widely used commercially in industry, biotechnology and health fields. Lipase enzyme functions to hydrolyze oils and fats by breaking them down into short-chain lipids and fatty acids. Lipase enzyme from bacteria is the most widely used because it has advantages such as being easy and fast to grow, cultivated, widely distributed so that it is easy to isolate anywhere and is relatively cheaper. This study aimed to determine the effect of corn oil inducer on the activity of lipase producing bacteria. The research method was carried out quantitatively using the titration method. The results showed that the highest lipase activity value using corn oil as inducer was in BPT6 isolate with a lipase activity value of 1.52 U/ml, in BP6 isolate with a lipase activity value of 1.37 U/ml. The lowest lipase activity value was demonstrated by isolate BP1 with 0.85 U/ml at 24 hours of incubation.