Abstract:
Miana (Coleus hybridus) is a species of the Lamiaceae family. This plant contains
medicinal compounds that can be used as pain management, anti-inflammatory,
antioxidant and antibacterial agents. Phytochemical tests showed that the green-red miana
leaves contain flavonoid, terpenoid, steroid, saponin, phenolic and alkaloid. This study
aimed to isolate secondary metabolites from green-red miana leaves and to test the
antioxidants activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Secondary
metabolite compounds from plants were isolated by maceration using methanol as solvent
and partitioned using n-hexane and ethyl acetate as solvents. Vacuum liquid
chromatography (VLC) and flash column chromatography were used to separated the
compounds in the viscous ethyl acetate fraction. Brown solids were obtained in vial 38 as
much as 17 mg which was coded as CHHM-EA-V6-FL38 and the thin layer
chromatography test still showed 2 spots on the plate. The CHHM-EA-V6-FL38
subfraction was characterized by FT-IR spectroscopy. Based on the IC50 results of the
CHHM-EA-V6-FL38 subfraction, it showed an IC50 value of 29.53 μg/mL at a
concentration of 100 g/mL which indicated that this subfraction could be concluded to be
active in the antioxidant test.
