SKRINING AKTIVITAS ANTIOKSIDAN DARI FRAKSI ETIL ASETAT Plectranthus ciliatus

Abstract

Plectranthus ciliatus is a plant that is easy to live in Indonesia and also is a native species from South Africa. Around 85% of Plectranthus were used in medicine. In addition, Plectranthus potentially has ability to produce secondary metabolites that are active against antioxidant activity. This study aimed to determine the secondary metabolite content of this plant and to test antioxidant activity of the extract and the ethyl acetate fraction of P. ciliatus. The results of phytochemical tests showed that the plant contained secondary metabolites such as alkaloids, steroids, saponins, phenolics and flavonoids. The extraction process was carried out using the maceration method with methanol as a solvent and continued with the fractionation process using the partition method to obtain a thick extract of ethyl acetate. Antioxidant testing using the DPPH method on the extract found that the ethyl acetate extract had strong antioxidant activity with an IC50 value of 115.34 μg/mL and the strongest ethyl acetate fraction was in the PC-E-V4-FL13 fraction with an IC50 value of 45.75 μg/ mL and PC-E-V7 fraction 55.78 g/mL.