ISOLASI DNA DAUN MANGGA KASTURI (Mangifera casturi K.) MENGGUNAKAN GENOMIC DNA MINI KIT (PLANT) GENEAID

Abstract

Mangifera casturi is an endemic mango from South Kalimantan that has become extinct in the wild and population tends to decrease. Information on the diversity of Mangifera casturi can be seen molecularly, considering that the Mangifera casturi is a plant that rarely appears so organs that are often found are incomplete for morphological analysis. Molecular analysis requires important steps, namely DNA isolation, because it will determine the quality and quantity of DNA to be used for further analysis. This study aimed to isolate the total DNA in 3 individual Kasturi mango plants with the help of a Genomic DNA plant minikit (Genaid). The isolation results showed that the total DNA of the Kasturi mango was successfully isolated using an isolation kit. The resulting total DNA has thick bands without smear.