IDENTIFIKASI MOLEKULER JAMUR TERMOFILIK Aspergillus sp. LBKURCC304 ISOLAT LOKAL BUKIK GADANG SUMATERA BARAT

Abstract

Fungi strain LBKURCC304 is a thermophilic fungi isolated from Bukik Gadang hot spring, West Sumatera, can produce amylase enzyme based on semi-quantitatively and quantitatively analysis. Morphological characteristics indicated that strain LBKURCC304 was a member of Aspergillus genera. Morphological identification cannot be used to determine species of fungi, therefore molecular identification is required. Determination of species is very important to explore the potential of LBKURCC304 with reference from the same species. Molecular identification of Aspergillus sp. LBKURCC304 was initiated by isolation of genomic DNA from 2 days old mycelia, PCR amplification on ITS-1 and ITS-2 rDNA regions were successfully performed using ITS4 and ITS5 primers at annealing temperatures of 45ºC. The sequences were verified with BioEdit program and then aligned to get the complete sequences. DNA sequences of Aspergillus sp. LBKURCC304 were analyzed using BLAST. The genomic DNA and PCR product have molecular weights of 16.425 bp and 593 bp, respectively. The BLAST analysis showed that Aspergillus sp. LBKURCC304 is Aspergillus fumigatus.