SELEKSI DAN UJI AKTIVITAS SELULASE ISOLAT BAKTERI ENDOFIT LBKURCC 392 s/d 418 DARI AKAR MANGROVE KABUPATEN BENGKALIS

Abstract

Endophytic bacteria from mangrove roots have the potential to produce cellulase enzymes. In this study, 27 isolates of endophytic bacteria from mangrove roots were selected from the collection of the Enzyme, Fermentation and Biomolecular Research Laboratory, Department of Chemistry, FMIPA UNRI. The selection was carried out on selective solid media of carboxyl methyl cellulose (CMC) 1%, while the crude extract enzyme activity was determined by the 3,5-dinitrosalicylic acid (DNS) method. The results of the selection of 27 isolates of endophytic bacteria from the Magrove roots of Bengkalis Regency on 1% CMC selective solid media obtained 22 live isolates because they were able to produce the cellulase enzyme and 5 isolates not alive. Statistical analysis with DNMRT at a significance level of 5% and PCA with the highest activity in the plant species Ceriops tagal (Perr.) C.B. Rob (LBKURCC 409) is (489 ±137) x 10-4 U/mL in 1st cluster and the lowest activity was in plant species Bruguiera sp. (LBKURCC 393) with an activity value of (28 ±17) x 10-4 U/mL in the 4th cluster.