Abstract:
The Laboratory of Enzyme, Fermentation and Biomolecular Research, Faculty of Mathematics
and Natural Sciences, Riau University has a collection of endophytic bacterial isolates from
Mangrove roots in Sei Pakning Jetty and Tenggayun Beach, Bengkalis Regency, Riau. This
isolate is thought to have the potential to produce an area of enzyme, therefore this study aimed
to select 28 isolates of cellulase-producing bacteria using 1% CMC selective solid media, while
for the production of cellulase enzymes in liquid media. Testing of cellulase enzyme activity was
carried out using the 3,5-Di Nitro Salissilic Acid (DNS) method. The enzyme activity data
obtained were then tested statistically using the Duncan's Multiple Range Test (DMNRT)
method at a significance level of 5% and Principal Component Analysis (PCA). The results of
the selection of 28 isolates of endophytic bacteria from the roots of the Bengkalis Regency on
1% CMC selective solid media obtained 26 isolates that were alive because they produced
cellulase enzymes and 2 were dead. The highest cellulase activity of the 13 crude extracts tested
was LBKURCC388 isolate (487±105) x 10-4 U/mL mangrove species Bruguiera sp. which is in
cluster 1, and the lowest activity is isolate LBKURCC365 mangrove plant species Ceriops tagal
(Perr.) C.B. Rob. with an activity value of (72±39) x 10-4 U/mL in the 4th cluster.
