OPTIMASI WAKTU PRODUKSI METABOLITE SEKUNDER AKTINOMISETES ASAL ARBORETUM UNIVERSITAS RIAU: UJI DAYA HAMBAT TERHADAP Escherichia coli dan Salmonella typhi

Abstract

Escherichia coli and Salmonella typhi is a gram negative, facultatively anaerobic bacteria and unable to form spores. The aim of this research is to determine the optimal time for actinomycetes arboretum riau university to inhibit growth of Escherichia coli and Salmonella thyphi that will bring disease inside digestive tract. The most common bacteria to cause infection in food are S.typhi and E.Coli. This research is done by adopting paper disc method alongside utilizing MHA medium. The optimum incubation period in producing secondary metabolite in isolate actinomycetes code B1.06,C2.18,C2.20 to Escherichia coli is on the 10th, 20th, 8th day of incubation period with inhibition on the 10th day 10,7 mm, 20th day 15,5 mm, 8th day 10,9 mm, the optimum time of the production of secondary metabolite in isolate actinomycetes code B1.06,C2.18,C2.20 to Salmonella typhi is on the 13th, 14th, 8th day with each inhibition on the 13th day 9,8 mm, 14th day 10,4 mm, 8th day 14,4 mm.