Abstract:
Polymerase Chain Reaction (PCR) is a technique that aims to amplify DNA in vitro.
Optimizing annealing temperature is one of the important factors that can determine the
success of the PCR process. This study aimed to determine the appropriate annealing
temperature for matK primers. The research procedure consisted of total DNA isolation,
PCR and electrophoresis. In this study, a pair of primers were used, namely matK-413f-
1 and matK-1227r-3. Optimization was carried out at three melting temperatures (Tm),
namely 52,3ºC, 50,5ºC, and 47,5ºC. Annealing temperature of 47,5ºC showed thick and
bright DNA bands with a size of about 900 bp. Based on these result, the appropriate
annealing temperature for the matK-413f-1 and matK-1227r-3 primer pair is 47,5ºC.